Statistics

 array:23 [
  "pii" => "S2013251423000834"
  "issn" => "20132514"
  "doi" => "10.1016/j.nefroe.2023.05.008"
  "estado" => "S300"
  "fechaPublicacion" => "2023-12-01"
  "aid" => "1149"
  "copyright" => "Sociedad Española de Nefrología"
  "copyrightAnyo" => "2023"
  "documento" => "article"
  "crossmark" => 1
  "subdocumento" => "fla"
  "cita" => "Nefrologia (English Version). 2023;43 Supl 2:38-46"
  "abierto" => array:3 [
    "ES" => true
    "ES2" => true
    "LATM" => true
  ]
  "gratuito" => true
  "lecturas" => array:1 [
    "total" => 0
  ]
  "itemSiguiente" => array:18 [
    "pii" => "S2013251423000846"
    "issn" => "20132514"
    "doi" => "10.1016/j.nefroe.2023.05.009"
    "estado" => "S300"
    "fechaPublicacion" => "2023-12-01"
    "aid" => "1150"
    "copyright" => "Sociedad Española de Nefrología"
    "documento" => "article"
    "crossmark" => 1
    "subdocumento" => "fla"
    "cita" => "Nefrologia (English Version). 2023;43 Supl 2:47-56"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:1 [
      "total" => 0
    ]
    "en" => array:12 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Original article</span>"
      "titulo" => "Serum chloride as a marker of cardiovascular and all-cause mortality in chronic hemodialysis patients&#58; 5-Year follow-up study"
      "tienePdf" => "en"
      "tieneTextoCompleto" => "en"
      "tieneResumen" => "en"
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "47"
          "paginaFinal" => "56"
        ]
      ]
      "contieneResumen" => array:1 [
        "en" => true
      ]
      "contieneTextoCompleto" => array:1 [
        "en" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "resumenGrafico" => array:2 [
        "original" => 0
        "multimedia" => array:7 [
          "identificador" => "fig0035"
          "etiqueta" => "Fig&#46; 2"
          "tipo" => "MULTIMEDIAFIGURA"
          "mostrarFloat" => true
          "mostrarDisplay" => false
          "figura" => array:1 [
            0 => array:4 [
              "imagen" => "gr2.jpeg"
              "Alto" => 2512
              "Ancho" => 2508
              "Tamanyo" => 352112
            ]
          ]
          "descripcion" => array:1 [
            "en" => "<p id="spar0055" class="elsevierStyleSimplePara elsevierViewall">Kaplan&#8211;Meier estimates of cumulative all-cause survival rates&#46; Patients were stratified into four groups according to median CRP and Cl<span class="elsevierStyleSup">&#8722;</span>&#46;</p>"
          ]
        ]
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "Francisco Valga, Tania Monzon, Nicanor Vega-Diaz, Angelo Santana, Giancarlo Moscol, Sergio Ruiz-Santana, Jose Carlos Rodriguez-Perez"
          "autores" => array:7 [
            0 => array:2 [
              "nombre" => "Francisco"
              "apellidos" => "Valga"
            ]
            1 => array:2 [
              "nombre" => "Tania"
              "apellidos" => "Monzon"
            ]
            2 => array:2 [
              "nombre" => "Nicanor"
              "apellidos" => "Vega-Diaz"
            ]
            3 => array:2 [
              "nombre" => "Angelo"
              "apellidos" => "Santana"
            ]
            4 => array:2 [
              "nombre" => "Giancarlo"
              "apellidos" => "Moscol"
            ]
            5 => array:2 [
              "nombre" => "Sergio"
              "apellidos" => "Ruiz-Santana"
            ]
            6 => array:2 [
              "nombre" => "Jose Carlos"
              "apellidos" => "Rodriguez-Perez"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2013251423000846?idApp=UINPBA000064"
    "url" => "/20132514/00000043000000S2/v2_202402210544/S2013251423000846/v2_202402210544/en/main.assets"
  ]
  "itemAnterior" => array:18 [
    "pii" => "S2013251423000822"
    "issn" => "20132514"
    "doi" => "10.1016/j.nefroe.2023.05.007"
    "estado" => "S300"
    "fechaPublicacion" => "2023-12-01"
    "aid" => "1146"
    "copyright" => "Sociedad Espa&#241;ola de Nefrolog&#237;a"
    "documento" => "article"
    "crossmark" => 1
    "subdocumento" => "fla"
    "cita" => "Nefrologia &#40;English Version&#41;. 2023;43 Supl 2:32-7"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:1 [
      "total" => 0
    ]
    "en" => array:13 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Original article</span>"
      "titulo" => "Nitrogen and phosphorus recovery from hemodialysis wastewater to use as an agricultural fertilizer"
      "tienePdf" => "en"
      "tieneTextoCompleto" => "en"
      "tieneResumen" => array:2 [
        0 => "en"
        1 => "es"
      ]
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "32"
          "paginaFinal" => "37"
        ]
      ]
      "titulosAlternativos" => array:1 [
        "es" => array:1 [
          "titulo" => "Recuperaci&#243;n de nitr&#243;geno y f&#243;sforo del agua residual de la hemodi&#225;lisis para utilizar como fertilizante agr&#237;cola"
        ]
      ]
      "contieneResumen" => array:2 [
        "en" => true
        "es" => true
      ]
      "contieneTextoCompleto" => array:1 [
        "en" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "resumenGrafico" => array:2 [
        "original" => 0
        "multimedia" => array:7 [
          "identificador" => "fig0010"
          "etiqueta" => "Fig&#46; 2"
          "tipo" => "MULTIMEDIAFIGURA"
          "mostrarFloat" => true
          "mostrarDisplay" => false
          "figura" => array:1 [
            0 => array:4 [
              "imagen" => "gr2.jpeg"
              "Alto" => 1368
              "Ancho" => 3175
              "Tamanyo" => 199847
            ]
          ]
          "descripcion" => array:1 [
            "en" => "<p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Effect of pH and temperature on phosphorus and nitrogen removal&#46;</p>"
          ]
        ]
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "Faissal Tarrass, Hamza Benjelloun, Meryem Benjelloun"
          "autores" => array:3 [
            0 => array:2 [
              "nombre" => "Faissal"
              "apellidos" => "Tarrass"
            ]
            1 => array:2 [
              "nombre" => "Hamza"
              "apellidos" => "Benjelloun"
            ]
            2 => array:2 [
              "nombre" => "Meryem"
              "apellidos" => "Benjelloun"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2013251423000822?idApp=UINPBA000064"
    "url" => "/20132514/00000043000000S2/v2_202402210544/S2013251423000822/v2_202402210544/en/main.assets"
  ]
  "en" => array:21 [
    "idiomaDefecto" => true
    "cabecera" => "<span class="elsevierStyleTextfn">Original article</span>"
    "titulo" => "ACSL1&#58; A preliminary study that provides a new target for the treatment of renal fibrosis could bring new insights in diabetic kidney disease"
    "tieneTextoCompleto" => true
    "paginas" => array:1 [
      0 => array:2 [
        "paginaInicial" => "38"
        "paginaFinal" => "46"
      ]
    ]
    "autores" => array:1 [
      0 => array:4 [
        "autoresLista" => "Zhonghui Cao, Xiao Gao, Jing Meng, Xiaoli Guo, Jiahao Xu, Junchao Cui, Xueyan Zhou"
        "autores" => array:7 [
          0 => array:3 [
            "nombre" => "Zhonghui"
            "apellidos" => "Cao"
            "referencia" => array:3 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">b</span>"
                "identificador" => "aff0010"
              ]
              2 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">1</span>"
                "identificador" => "fn0005"
              ]
            ]
          ]
          1 => array:3 [
            "nombre" => "Xiao"
            "apellidos" => "Gao"
            "referencia" => array:2 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">1</span>"
                "identificador" => "fn0005"
              ]
            ]
          ]
          2 => array:3 [
            "nombre" => "Jing"
            "apellidos" => "Meng"
            "referencia" => array:2 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">1</span>"
                "identificador" => "fn0005"
              ]
            ]
          ]
          3 => array:3 [
            "nombre" => "Xiaoli"
            "apellidos" => "Guo"
            "referencia" => array:2 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">1</span>"
                "identificador" => "fn0005"
              ]
            ]
          ]
          4 => array:3 [
            "nombre" => "Jiahao"
            "apellidos" => "Xu"
            "referencia" => array:2 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">1</span>"
                "identificador" => "fn0005"
              ]
            ]
          ]
          5 => array:3 [
            "nombre" => "Junchao"
            "apellidos" => "Cui"
            "referencia" => array:2 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">1</span>"
                "identificador" => "fn0005"
              ]
            ]
          ]
          6 => array:4 [
            "nombre" => "Xueyan"
            "apellidos" => "Zhou"
            "email" => array:1 [
              0 => "zxy851107@xzhmu.edu.cn"
            ]
            "referencia" => array:3 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">1</span>"
                "identificador" => "fn0005"
              ]
              2 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#42;</span>"
                "identificador" => "cor0005"
              ]
            ]
          ]
        ]
        "afiliaciones" => array:2 [
          0 => array:3 [
            "entidad" => "Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy&#44; College of Pharmacy&#44; Xuzhou Medical University&#44; Xuzhou 221004&#44; China"
            "etiqueta" => "a"
            "identificador" => "aff0005"
          ]
          1 => array:3 [
            "entidad" => "Department of Pharmacy&#44; Affiliated Hospital of Xuzhou Medical University&#44; Xuzhou 221004&#44; China"
            "etiqueta" => "b"
            "identificador" => "aff0010"
          ]
        ]
        "correspondencia" => array:1 [
          0 => array:3 [
            "identificador" => "cor0005"
            "etiqueta" => "&#8270;"
            "correspondencia" => "<span class="elsevierStyleItalic">Corresponding author</span>&#46;"
          ]
        ]
      ]
    ]
    "titulosAlternativos" => array:1 [
      "es" => array:1 [
        "titulo" => "ACSL1&#58; un estudio preliminar que aporta un nuevo objetivo para el tratamiento de la fibrosis renal podr&#237;a proporcionar una nueva percepci&#243;n en la nefropat&#237;a diab&#233;tica"
      ]
    ]
    "resumenGrafico" => array:2 [
      "original" => 0
      "multimedia" => array:7 [
        "identificador" => "fig0020"
        "etiqueta" => "Fig&#46; 4"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr4.jpeg"
            "Alto" => 2081
            "Ancho" => 3258
            "Tamanyo" => 302054
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">Effects of ACSL1 knockdown on renal fibrosis and EMT in HK-2 cells&#46; &#40;A&#41; WB analysis demonstrated the expression levels of ACSL1 in HK-2 cells&#46; &#40;B&#41; Quantitation of the arbitrary ratios of ACSL1 to GAPDH&#46; &#40;C&#41; WB analysis demonstrated the expression levels of EMT-related proteins in HK-2 cells&#46; &#40;D&#41; Quantitation of the arbitrary ratios of EMT-related proteins to GAPDH&#46; HG&#58; cells treated with 60<span class="elsevierStyleHsp" style=""></span>mmol&#47;L glucose&#59; HG<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>NC&#58; cells treated with HG and transfected with NC lentivirus&#59; and HG<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>ACSL1-shRNA&#58; cells treated with HG and transfected with ACSL1-shRNA lentivirus&#46; Data information&#58; all values are shown as the means<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>SEMs of at least three independent experiments&#46; &#42;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#44; &#42;&#42;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;01&#44; mean HG<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>ACSL1-shRNA compared with HG&#46;</p>"
        ]
      ]
    ]
    "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0065">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">Diabetic kidney disease &#40;DKD&#41; is the main cause of end-stage renal disease&#46;<a class="elsevierStyleCrossRef" href="#bib0190"><span class="elsevierStyleSup">1</span></a> The mechanisms leading to DKD are complex&#44; and tubulointerstitial fibrosis is a common pathway for most renal injuries<a class="elsevierStyleCrossRef" href="#bib0195"><span class="elsevierStyleSup">2</span></a> and one of the main pathological features of chronic kidney disease&#46;<a class="elsevierStyleCrossRef" href="#bib0200"><span class="elsevierStyleSup">3</span></a> Kidney fibroblasts play an important role in maintaining the homeostasis of the interstitial matrix and adjacent tissues under physiological conditions&#46; Many cells are involved in the process of renal fibrosis&#46; The main cause of tubulointerstitial fibrosis is the activation and expansion of fibroblasts&#44; the production and deposition of a large number of extracellular matrix &#40;ECM&#41; components&#44; and changes in both renal tubules and microvessels&#46;<a class="elsevierStyleCrossRefs" href="#bib0205"><span class="elsevierStyleSup">4&#44;5</span></a> Continued exposure to a high glucose &#40;HG&#41; environment induces epithelial-mesenchymal transition &#40;EMT&#41; in renal tubular epithelial cells&#44; which in turn leads to interstitial fibrosis<a class="elsevierStyleCrossRef" href="#bib0215"><span class="elsevierStyleSup">6</span></a> and the accumulation of extracellular matrix proteins such as collagen in the mesangial interstitium&#46; The accumulation of protein and fibronectin leads to tubulointerstitial fibrosis&#46;<a class="elsevierStyleCrossRefs" href="#bib0220"><span class="elsevierStyleSup">7&#44;8</span></a> At present&#44; the control of fibrosis development involves preventing&#44; alleviating&#44; and even reversing DKD by addressing the interaction between hemodynamic and metabolic pathways<a class="elsevierStyleCrossRef" href="#bib0230"><span class="elsevierStyleSup">9</span></a> and controlling blood sugar&#44; blood pressure and blood lipids using the renin&#8211;angiotensin&#8211;aldosterone system&#46; The main drugs that block the action and can treat dyslipidemia are also effective agents for preventing diabetic nephropathy&#46;<a class="elsevierStyleCrossRef" href="#bib0235"><span class="elsevierStyleSup">10</span></a> However&#44; many patients develop DKD despite the control of blood sugar&#44; lipids&#44; and blood pressure&#46;<a class="elsevierStyleCrossRefs" href="#bib0240"><span class="elsevierStyleSup">11&#44;12</span></a> By 2040&#44; the prevalence of diabetes is expected to exceed 600 million&#44; of which more than 20 million will develop DKD&#46;<a class="elsevierStyleCrossRef" href="#bib0250"><span class="elsevierStyleSup">13</span></a> Therefore&#44; it is urgent to explore potential therapeutic targets for DKD to slow down the occurrence and development of DKD&#46;</p><p id="par0010" class="elsevierStylePara elsevierViewall">Acyl-CoA synthase long-chain family member 1 &#40;long-chain-fatty-acid&#8211;CoA ligase 1&#44; ACSL1&#41; is an enzyme in the initial step of fatty acid &#946;-oxidation and plays a key role in lipid biosynthesis and fatty acid degradation&#46; Fatty acids are essential nutrients for the human body and can be obtained from diet or from de novo synthesis and breakdown of cellular triacylglycerols &#40;TAGs&#41; and phospholipids&#46; As an important part of the body&#44; these factors are also involved in energy metabolism and cell signaling pathways to maintain physiological functions&#46; Long-chain acyl-CoA synthase &#40;ACSL&#41; activates fatty acids for intracellular metabolism and is also involved in the regulation of uptake&#46;<a class="elsevierStyleCrossRef" href="#bib0255"><span class="elsevierStyleSup">14</span></a> However&#44; dysregulation of fatty acid metabolism leads to excessive lipid biosynthesis and deposition&#44; which ultimately predisposes the body to metabolic disorders&#46;<a class="elsevierStyleCrossRef" href="#bib0260"><span class="elsevierStyleSup">15</span></a> Some studies have shown that ACSL1 is directly involved in lipid deposition in the kidney&#46;<a class="elsevierStyleCrossRef" href="#bib0265"><span class="elsevierStyleSup">16</span></a> In addition&#44; in a study of colorectal cancer&#44; it was found that ACSL1 could cause the transition of epithelial cells to mesenchymal cells through the AMPK signaling pathway&#46; At the same time&#44; the markers N-cadherin and Slug were upregulated&#44; suggesting that ACSL1 could serve as a direct therapeutic target for colorectal cancer&#46;<a class="elsevierStyleCrossRef" href="#bib0270"><span class="elsevierStyleSup">17</span></a> However&#44; ACSL1 has been poorly studied in diabetic kidney disease&#46;</p><p id="par0015" class="elsevierStylePara elsevierViewall">In addition&#44; the use of urine proteomics and bioinformatics can provide insight into diabetic kidney disease&#44;<a class="elsevierStyleCrossRef" href="#bib0275"><span class="elsevierStyleSup">18</span></a> and it is also possible to predict the occurrence and development of DKD by using biomarkers in urine&#46;<a class="elsevierStyleCrossRefs" href="#bib0280"><span class="elsevierStyleSup">19&#44;20</span></a> GSK3b in exfoliated cells may serve as a novel biomarker for predicting the progression of diabetic kidney disease&#46;<a class="elsevierStyleCrossRef" href="#bib0290"><span class="elsevierStyleSup">21</span></a> Urine represents an infinite source of patient-specific kidney cells that can be obtained noninvasively&#46; Podocytes and proximal tubule cells in urine have been used to investigate disease mechanisms and screen for novel drug therapies for various human kidney diseases&#46;<a class="elsevierStyleCrossRef" href="#bib0295"><span class="elsevierStyleSup">22</span></a></p><p id="par0020" class="elsevierStylePara elsevierViewall">In the present study&#44; we found that ACSL1 was significantly upregulated in the urine and kidney tissue of diabetic kidney disease patients and db&#47;db mice&#46; Knockdown of ACSL1 under HG conditions significantly alleviated renal fibrosis&#46; Therefore&#44; ACSL1 may be used as a therapeutic target to prevent or ameliorate the progression of DKD fibrosis&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Experimental procedure</span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Clinical studies</span><p id="par0025" class="elsevierStylePara elsevierViewall">This study was conducted at the Affiliated Hospital of Xuzhou Medical University from March to June 2022&#46; A total of 84 urine samples were collected&#44; including 20 urine samples from healthy people at the Health Management Center of the Affiliated Hospital of Xuzhou Medical University&#59; urine samples were collected from 30 diabetic patients and 34 diabetic nephropathy patients in the Department of Endocrinology&#46; Each individual&#39;s basic information and clinical parameters were recorded&#44; including sex&#44; age&#44; medication&#44; diabetes duration&#44; blood pressure&#44; blood sugar level&#44; serum creatinine level&#44; urine analysis data&#44; UACR and eGFR&#46; All patients provided informed consent&#46;</p><p id="par0030" class="elsevierStylePara elsevierViewall">Diagnostic criteria present for DKD are usually based on persistence albuminuria and&#47;or eGFR levels decreased&#44; while other sources were excluded a clinical diagnosis was made of induced chronic kidney disease &#40;CKD&#41;&#46; In defining diabetes as kidney etiology of lesions and exclusion of other causes of CKD in the case of to less than one of the following can be diagnosed as DKD&#58; &#40;1&#41; eliminate interference factors at least 2 out of 3 tests within 3&#8211;6 months UACR<span class="elsevierStyleHsp" style=""></span>&#8805;<span class="elsevierStyleHsp" style=""></span>30<span class="elsevierStyleHsp" style=""></span>mg&#47;g or urinary albumin excretion rate &#8805;30<span class="elsevierStyleHsp" style=""></span>mg&#47;24<span class="elsevierStyleHsp" style=""></span>h &#40;&#8805;20<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;min&#41;&#59; &#40;2&#41; eGFR<span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>60<span class="elsevierStyleHsp" style=""></span>mL&#47;&#40;min 1&#46;73<span class="elsevierStyleHsp" style=""></span>m<span class="elsevierStyleSup">2</span>&#41; lasted 3 times more than a month&#59; &#40;3&#41; renal biopsy was consistent with the pathological changes of DKD&#46;<a class="elsevierStyleCrossRef" href="#bib0300"><span class="elsevierStyleSup">23</span></a></p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Urine samples</span><p id="par0035" class="elsevierStylePara elsevierViewall">A 10<span class="elsevierStyleHsp" style=""></span>mL urine sample from the first morning urine stream was collected&#46; Urine samples were centrifuged at 3000<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">g</span> for 15<span class="elsevierStyleHsp" style=""></span>min and stored at &#8722;80<span class="elsevierStyleHsp" style=""></span>&#176;C&#46; The supernatant was divided into 1&#46;5<span class="elsevierStyleHsp" style=""></span>mL portions and immediately stored at &#8722;80<span class="elsevierStyleHsp" style=""></span>&#176;C&#46; The urine samples were centrifuged at 10&#44;000<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">g</span> for 15<span class="elsevierStyleHsp" style=""></span>min&#44; and the exfoliated cells were retained and immediately stored at &#8722;80<span class="elsevierStyleHsp" style=""></span>&#176;C&#46;</p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">ELISA</span><p id="par0040" class="elsevierStylePara elsevierViewall">A specific ELISA kit was used to detect the expression of ACSL1 in the urine of healthy subjects&#44; diabetic patients and diabetic kidney disease patients&#46;</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0090">Animals</span><p id="par0045" class="elsevierStylePara elsevierViewall">Twelve male C57BLKS&#47;J mice with a Lep db&#47;Lep db &#40;db&#47;db&#41; &#40;7 weeks old&#44; 30&#8211;35<span class="elsevierStyleHsp" style=""></span>g&#41; background and nondiabetic control Lep db&#47;m &#40;db&#47;m&#41; littermate mice &#40;7 weeks old&#44; 15&#8211;20<span class="elsevierStyleHsp" style=""></span>g&#41; were purchased from the Model Animal Research Center of Nanjing University &#40;Jiangsu&#44; China&#41;&#46; All animals were housed in a barrier environment and provided food and water ad libitum before and during the experiments&#46; All experimental procedures were approved by the Animal Ethics Committee of Xuzhou Medical University&#46; Some db&#47;m and db&#47;db mice were fed until they were 24 weeks old&#44; and blood&#44; urine and kidney tissues were collected to observe the expression of ACSL1 in the kidneys of diabetic nephropathy mice&#46;</p></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0095">Detection of renal function and biochemical parameters</span><p id="par0050" class="elsevierStylePara elsevierViewall">Renal function was assessed through the measurement of blood urea nitrogen &#40;BUN&#41;&#44; blood creatinine &#40;Cr&#41; and urinary albumin levels&#46; BUN and Cr were determined using assay kits from Jiancheng &#40;Nanjing&#44; China&#41;&#46; Serum levels of fasting blood glucose &#40;FBG&#41; were measured by test strips from LifeScan &#40;CA&#44; USA&#41;&#46; The kidney index &#40;mg&#47;g&#41; was calculated as the ratio of the weight of the two kidneys to the body weight of the mouse&#46;</p></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0100">Cell culture and transfection</span><p id="par0055" class="elsevierStylePara elsevierViewall">The human proximal renal tubular epithelial cell line HK-2 was a gift from Nanjing University&#46; The cells were cultured in DMEM containing 5&#46;56<span class="elsevierStyleHsp" style=""></span>mmol&#47;L glucose &#40;normal glucose&#44; NG&#41; supplemented with 10&#37; fetal bovine serum &#40;Invitrogen&#44; Grand Island&#44; NY&#41; and 1&#37; penicillin&#8211;streptomycin in a humidified incubator at 37<span class="elsevierStyleHsp" style=""></span>&#176;C with 5&#37; CO<span class="elsevierStyleInf">2</span>&#46; To induce EMT&#44; the cells were cultured in medium containing 60<span class="elsevierStyleHsp" style=""></span>mmol&#47;L glucose for 72<span class="elsevierStyleHsp" style=""></span>h&#46; Glucose &#40;5&#46;56<span class="elsevierStyleHsp" style=""></span>mmol&#47;L&#41; and mannitol were used for osmotic control &#40;MA&#41;&#46;</p><p id="par0060" class="elsevierStylePara elsevierViewall">For ACSL1-shRNA experiments&#44; HK-2 cells were stimulated with HG and transfected with the control lentivirus vector &#40;HG<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>NC&#41; or ACSL1<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>shRNA lentivirus vector &#40;HG<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>ACSL1-shRNA&#41; for 72<span class="elsevierStyleHsp" style=""></span>h&#46; HK-2 cells were cultured in HG without other treatments &#40;HG&#41; as a control&#46;</p></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0105">Histopathological analysis</span><p id="par0065" class="elsevierStylePara elsevierViewall">Kidney tissues were fixed in 10&#37; phosphate buffered formalin and embedded in paraffin&#46; Paraffin sections &#40;2&#8211;3<span class="elsevierStyleHsp" style=""></span>&#956;m&#41; were stained with periodic acid-Schiff &#40;PAS&#41; and Masson&#39;s trichrome&#46;</p></span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0110">Histology and immunohistochemistry analysis</span><p id="par0070" class="elsevierStylePara elsevierViewall">The kidney samples were fixed in 4&#37; paraformaldehyde and embedded in paraffin&#46; Sections with a thickness of 5<span class="elsevierStyleHsp" style=""></span>&#956;m were cut perpendicular to the long axis of the kidney for immunohistochemistry and morphometric analysis&#46;</p></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0115">Western blot analyses</span><p id="par0075" class="elsevierStylePara elsevierViewall">Urinary exfoliated cells were lysed with ice-cold RIPA buffer&#44; and the insoluble materials were removed&#46; The proteins were separated by 10&#37; sodium dodecyl sulfate-polyacrylamide &#40;SDS&#41; gel electrophoresis&#44; transferred onto nitrocellulose &#40;NC&#41; membranes and blocked with 3&#37; BSA for 2<span class="elsevierStyleHsp" style=""></span>h at RT&#46; The membranes were incubated with primary antibodies at 4<span class="elsevierStyleHsp" style=""></span>&#176;C overnight and then with horseradish peroxidase-conjugated secondary antibodies for 1<span class="elsevierStyleHsp" style=""></span>h&#46; The primary antibody used was anti-ACSL1 &#40;Affinity&#41;&#46; The intensities of the bands were quantified using an Odyssey&#174; Sa system &#40;LICOR&#44; USA&#41;&#46; Protein signals were quantified by optical density ratios using GAPDH expression as a control&#46;</p></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0120">Statistical analysis</span><p id="par0080" class="elsevierStylePara elsevierViewall">Statistical analyses were performed using SPSS statistics 20&#46;0 software&#44; and all experimental data are expressed as the mean<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>SEM&#46; Comparisons among groups were performed using one-way analysis of variance &#40;ANOVA&#41;&#46; Differences between two groups were assessed with Student&#39;s <span class="elsevierStyleItalic">t</span> test&#46; Differences were considered significant if <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#46;</p></span></span><span id="sec0065" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0125">Results</span><span id="sec0070" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0130">Basic clinical data and biochemical indicators</span><p id="par0085" class="elsevierStylePara elsevierViewall">First&#44; the basic clinical information of the subjects was compared&#46; The results showed that there were no significant differences in age&#44; sex&#44; body mass index &#40;BMI&#41;&#44; low-density lipoprotein cholesterol &#40;LDL-C&#41;&#44; triglycerides &#40;TGs&#41;&#44; or total cholesterol &#40;TC&#41; among the subjects&#46; However&#44; there were significant differences in fasting blood glucose&#44; diastolic blood pressure&#44; and high-density lipoprotein cholesterol &#40;HDL-C&#41; in DM patients compared with controls &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#44; <a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46; Compared with DM patients&#44; there were also significant differences in fasting blood glucose&#44; C2h&#44; INS0h&#44; and INS1h in DKD patients &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#44; <a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#41;&#46; In addition&#44; in terms of clinical renal function indicators&#44; there were significant differences in IGU&#44; MA&#44; &#945;1-M&#44; UACR&#44; blood Cys C&#44; Scr and eGFR in DKD patients compared with DM patients &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#44; <a class="elsevierStyleCrossRef" href="#tbl0015">Table 3</a>&#41;&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><elsevierMultimedia ident="tbl0010"></elsevierMultimedia><elsevierMultimedia ident="tbl0015"></elsevierMultimedia></span><span id="sec0075" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0135">ACSL1 is overexpressed and closely related to the clinical renal function index of DKD patients</span><p id="par0090" class="elsevierStylePara elsevierViewall">Subsequently&#44; the expression levels of ACSL1 in the urine exfoliated cells of the different test groups were detected by WB analysis&#46; Compared with the NC group and the DM group&#44; the expression level of ACSL1 in the urinary exfoliated cells in the DKD group was significantly increased &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>A&#41;&#46; The expression levels of ACSL1 in the urine supernatants of the different test groups were detected by ELISA&#46; Compared with the NC group and the DM group&#44; the expression level of ACSL1 in the urinary supernatant of the DKD group was also significantly increased &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>B&#41;&#46; More notably&#44; the upregulation of ACSL1 in the urine of DKD patients was positively correlated with UACR &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>C&#41;&#44; immunoglobulin G &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>D&#41;&#44; and cystatin C &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>E&#41; and negatively correlated with eGFR &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>F&#41;&#46; These results suggest that ACSL1 may be involved in the pathogenesis of DKD&#46;</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia></span><span id="sec0080" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0140">ACSL1 expression is increased in db&#47;db mice</span><p id="par0095" class="elsevierStylePara elsevierViewall">To investigate the effect of HG on ACSL1 expression in vivo&#44; we constructed a db&#47;db mouse model and conducted experiments&#44; urinary microalbumin to creatinine &#40;mAlb&#47;Cr&#41; levels and fasting blood glucose &#40;FBG&#41; levels in db&#47;db mice were markedly elevated compared with those in db&#47;m mice&#46; Compared with db&#47;m mice&#44; PAS staining showed that renal tubular dilatation was more obvious in db&#47;db mice&#44; indicating renal tubular injury &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>A&#41;&#46; Compared with db&#47;m mice&#44; the distribution of Masson-positive areas was significantly increased in db&#47;db mice&#44; showing collagen accumulation in the renal cortex &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>B&#41;&#46; The results showed that renal function was decreased in diabetic mice&#46; In addition&#44; the expression of total ACSL1 protein was significantly increased in db&#47;db mice &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>C and D&#41;&#44; and immunohistochemistry showed that this expression was more pronounced around renal tubules &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>E&#41;&#46; These results suggest that ACSL1 may be involved in the occurrence of diabetic kidney disease in mice&#46;</p><elsevierMultimedia ident="fig0010"></elsevierMultimedia></span><span id="sec0085" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0145">The expression of ACSL1 is regulated by HG in HK-2 cells and promotes renal fibrosis</span><p id="par0100" class="elsevierStylePara elsevierViewall">Then&#44; we validated these changes in vitro&#46; HK-2 cells were treated with HG medium &#40;60<span class="elsevierStyleHsp" style=""></span>mmol&#47;L <span class="elsevierStyleSmallCaps">d</span>-glucose&#41; for 72<span class="elsevierStyleHsp" style=""></span>h&#46; The results showed that HG promoted the expression of ACSL1 &#40;<a class="elsevierStyleCrossRef" href="#fig0015">Fig&#46; 3</a>A and B&#41;&#44; and to eliminate the effect of HG-induced osmotic changes&#44; mannitol was used as an osmotic control&#46; In addition&#44; HG inhibited the expression of E-cadherin and Zo-1 and increased the expression of &#945;-SMA and Vimentin&#44; which are mesenchymal proteins&#46; These data suggest that HG induces EMT in HK-2 cells&#46;</p><elsevierMultimedia ident="fig0015"></elsevierMultimedia></span><span id="sec0090" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0150">ACSL1 knockdown reverses renal fibrosis in HG-induced HK-2 cells</span><p id="par0105" class="elsevierStylePara elsevierViewall">To further clarify the role of ACSL1 in EMT during DKD&#44; we reduced the protein expression of ACSL1 in HG-induced HK-2 cells &#40;<a class="elsevierStyleCrossRef" href="#fig0020">Fig&#46; 4</a>A and B&#41; and found that the expression of epithelial cell markers &#40;E-cadherin and ZO-1&#41; was significantly upregulated&#46; The expression of mesenchymal cell markers &#40;vimentin and &#945;-SMA&#41; was downregulated &#40;<a class="elsevierStyleCrossRef" href="#fig0020">Fig&#46; 4</a>C and D&#41;&#46; Taken together&#44; these results suggest that ACSL1 knockout can effectively improve EMT during renal fibrosis induced by diabetic kidney disease&#46;</p><elsevierMultimedia ident="fig0020"></elsevierMultimedia></span></span><span id="sec0095" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0155">Discussion</span><p id="par0110" class="elsevierStylePara elsevierViewall">Diabetic kidney disease &#40;DKD&#41; is the main cause of end-stage renal disease&#44;<a class="elsevierStyleCrossRef" href="#bib0190"><span class="elsevierStyleSup">1</span></a> which is one of the most common and serious complications of diabetes mellitus &#40;DM&#41;&#46;<a class="elsevierStyleCrossRef" href="#bib0305"><span class="elsevierStyleSup">24</span></a> There are many factors that cause diabetic nephropathy&#44; and its pathogenesis is complicated&#46; At present&#44; its pathogenesis is not fully understood&#44; which leads to poor treatment outcomes&#46; Standard treatment mainly controls blood glucose&#44; blood pressure and blood lipids&#46; However&#44; despite the control of blood glucose&#44; blood pressure and blood lipids&#44; many patients still develop DKD&#46;<a class="elsevierStyleCrossRef" href="#bib0240"><span class="elsevierStyleSup">11</span></a> In recent years&#44; an increasing number of studies have attempted to find therapeutic targets for DKD to delay or control the occurrence and development of DKD&#46;</p><p id="par0115" class="elsevierStylePara elsevierViewall">Urine is produced by glomerular filtration and reabsorption in the renal tubules&#46; The exfoliated renal cells in urine contain biological information and can also reflect the degree of renal tissue damage&#46;<a class="elsevierStyleCrossRef" href="#bib0310"><span class="elsevierStyleSup">25</span></a> Urine specimens are noninvasive and readily available&#44; and the use of urine proteomics and bioinformatics analyses provides insights into diabetic kidney disease&#46;<a class="elsevierStyleCrossRef" href="#bib0275"><span class="elsevierStyleSup">18</span></a> In the present study&#44; we measured the expression of ACSL1 in urine exfoliated cells by WB and detected the expression of ACSL1 in urine supernatants by ELISA&#46; Compared with that in healthy and diabetic patients&#44; the expression of ACSL1 in the urine of DKD patients was significantly higher &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>A and B&#41;&#44; which was closely related to renal function in DKD &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>D&#8211;F&#41;&#46; Therefore&#44; we hypothesize that ACSL1 may be involved in the occurrence and development of DKD&#46; However&#44; the small sample size was a limitation of the study&#46; In this study&#44; we only enrolled 84 patients based on our inclusion criteria for healthy individuals&#44; diabetic patients&#44; and diabetic nephropathy&#44; so we did not have sufficient statistical power to detect the effect of ACSL1 on some clinical indicators&#46;</p><p id="par0120" class="elsevierStylePara elsevierViewall">Long-chain acyl-CoA synthase &#40;ACSL&#41; activates fatty acids for intracellular metabolism and participates in the regulation of uptake&#46;<a class="elsevierStyleCrossRef" href="#bib0255"><span class="elsevierStyleSup">14</span></a> However&#44; dysregulation of fatty acid metabolism leads to excessive lipid biosynthesis and deposition&#44; which ultimately makes the body prone to metabolic disorders&#46;<a class="elsevierStyleCrossRef" href="#bib0260"><span class="elsevierStyleSup">15</span></a> ACSL1 also plays a very important role in regulating TG synthesis in the liver&#46;<a class="elsevierStyleCrossRef" href="#bib0315"><span class="elsevierStyleSup">26</span></a> Studies have shown that microRNA-34A and miR-34C target ACSL1 and reduce the expression of ACSL1&#44; thus reducing the expression of &#945;-SMA&#44; type I collagen and desmin and alleviating liver fibrosis&#44;<a class="elsevierStyleCrossRefs" href="#bib0320"><span class="elsevierStyleSup">27&#44;28</span></a> indicating that the overexpression of ACSL1 promotes liver fibrosis&#46; Therefore&#44; we considered whether the overexpression of ACSL1 promoted the occurrence and development of DKD-induced renal fibrosis&#46;</p><p id="par0125" class="elsevierStylePara elsevierViewall">Renal interstitial fibrosis is an important pathological basis for the development of diabetic nephropathy&#46; Compared with glomerulosclerosis&#44; renal interstitial fibrosis can more accurately reflect the degree of kidney damage&#44; which leads to renal failure&#46; Although the mechanism of renal fibrosis is still unclear&#44; increasing evidence indicates that epithelial-mesenchymal transition &#40;EMT&#41; plays an important role in the process of renal fibrosis&#46;<a class="elsevierStyleCrossRef" href="#bib0330"><span class="elsevierStyleSup">29</span></a> EMT can occur under various conditions&#44; such as in an animal model of renal fibrosis&#46;<a class="elsevierStyleCrossRef" href="#bib0335"><span class="elsevierStyleSup">30</span></a> During EMT&#44; epithelial cells lose characteristic markers&#44; such as E-cadherin and cytokeratin&#44; and gain mesenchymal cell-specific morphological and molecular markers&#44; including &#945;-smooth muscle actin &#40;&#945;-SMA&#41;&#44; fibronectin &#40;FN&#41;&#44; and type I collagen&#46;<a class="elsevierStyleCrossRef" href="#bib0340"><span class="elsevierStyleSup">31</span></a> Epithelial cells with EMT do not directly generate interstitial myofibroblasts&#46; Instead&#44; these cells release signals that promote the transformation of fibroblasts into myofibroblasts&#46; EMT describes a complex phenotypic change in epithelial cells&#46;<a class="elsevierStyleCrossRef" href="#bib0345"><span class="elsevierStyleSup">32</span></a> Therefore&#44; we constructed a db&#47;db mouse model and used PAS and Masson staining to show that the kidneys of db&#47;db mice showed obvious renal tubular injury and fibrosis compared with those of db&#47;m mice &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>A and B&#41;&#46; The expression of ACSL1 was upregulated in db&#47;db mouse renal tissues&#44; as shown by WB&#44; and the expression of ACSL1 around renal tubules was also significantly upregulated&#44; as shown by immunohistochemistry &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>C&#8211;E&#41;&#46; These results suggest that ACSL1 may play an important role in DKD-induced renal fibrosis&#46;</p><p id="par0130" class="elsevierStylePara elsevierViewall">Studies have shown that DKD is closely related to fibrosis in renal tubular epithelial cells&#46;<a class="elsevierStyleCrossRef" href="#bib0350"><span class="elsevierStyleSup">33</span></a> Epithelial cells&#44; which are located in the outer layer of the renal tubule&#44; are responsible for reabsorbing glucose&#44; amino acids and other substances in urine&#46;<a class="elsevierStyleCrossRef" href="#bib0355"><span class="elsevierStyleSup">34</span></a> Early studies have shown that exposure to high glucose or albumin levels can induce fibrosis in renal tubule epithelial cells&#46; This effect is closely related to the increased expression of McP-1&#44; PAI-1 and TGF-&#946;1 due to ROS production induced by hyperglycemia&#46;<a class="elsevierStyleCrossRef" href="#bib0360"><span class="elsevierStyleSup">35</span></a> In addition&#44; fibrosis in renal tubular epithelial cells is closely related to proteinuria&#46;<a class="elsevierStyleCrossRef" href="#bib0365"><span class="elsevierStyleSup">36</span></a> Hyperglycemia can also cause renal tubular epithelial cells to lose polarity and acquire migration and invasion characteristics&#44;<a class="elsevierStyleCrossRef" href="#bib0370"><span class="elsevierStyleSup">37</span></a> leading to increased expression of fibronectin and &#945;-smooth muscle actin &#40;&#945;-SMA&#41; and decreased expression of E-cadherin&#44; which ultimately lead to fibrosis&#46; We stimulated HK-2 cells with an HG concentration of 60<span class="elsevierStyleHsp" style=""></span>mmol&#47;L for 72<span class="elsevierStyleHsp" style=""></span>h&#44; and Western blotting showed that ACSL1 expression was increased by HG compared with NG &#40;<a class="elsevierStyleCrossRef" href="#fig0015">Fig&#46; 3</a>A and B&#41;&#46; Moreover&#44; EMT-related proteins and the epithelial cell markers ZO-1 and E-cadherin&#44; were downregulated&#44; and the mesenchymal cell markers vimentin and &#945;-SMA were upregulated &#40;<a class="elsevierStyleCrossRef" href="#fig0015">Fig&#46; 3</a>C and D&#41;&#44; which was consistent with other studies&#46; To further verify that these observed changes were the result of HG itself and not of HG-induced osmotic pressure changes&#44; an osmotic pressure control was included in our study&#44; and the results excluded the possibility of in vitro osmotic pressure changes&#46;</p><p id="par0135" class="elsevierStylePara elsevierViewall">Subsequently&#44; we hypothesized that decreased ACSL1 expression could inhibit renal fibrosis and contribute to the treatment of DKD-induced renal fibrosis&#46; We conducted further studies of HK-2 cells to reveal the role of ACSL1 in EMT during DKD&#46; The recovery of EMT-related protein expression in cells treated with HG and ACSL1-shRNA confirmed our previous hypothesis &#40;<a class="elsevierStyleCrossRef" href="#fig0020">Fig&#46; 4</a>C and D&#41;&#46; Therefore&#44; our data suggest that ACSL1 may be a new therapeutic target for DKD-associated renal fibrosis&#46;</p><p id="par0140" class="elsevierStylePara elsevierViewall">In conclusion&#44; we found a potential therapeutic target for preventing or ameliorating the progression of DKD-associated fibrosis&#46; We found that the expression of ACSL1 was significantly increased in the urine of patients with DKD&#46; Then&#44; by constructing a db&#47;db mouse model&#44; we found that severe renal fibrosis occurred in mice&#44; and the expression of ACSL1 was significantly increased in kidney tissue&#46; ACSL1 expression was also increased in HK-2 cells in response to HG stimulation&#46; In addition&#44; gene-specific downregulation of ACSL1 expression could reverse HG-induced EMT&#46; Therefore&#44; targeting ACSL1 may be a promising therapeutic strategy for the treatment of DKD&#44; renal fibrosis and other metabolism-related fibrotic diseases&#46; In addition&#44; these results may pave the way for large-scale&#44; in-depth studies of ACSL1 as a therapeutic target for DKD and renal fibrosis&#46;</p></span><span id="sec0100" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0160">Conclusion</span><p id="par0145" class="elsevierStylePara elsevierViewall">In summary&#44; ACSL1 may become a target for research for the treatment of diabetic nephropathy fibrosis&#44; and our results provide an effective and accurate experimental basis for the research of new drugs&#46;</p></span><span id="sec0105" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0165">Availability of data and materials</span><p id="par0150" class="elsevierStylePara elsevierViewall">The data that support the findings of this study are available from the corresponding author upon reasonable request&#46;</p></span><span id="sec0110" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0170">Author contributions</span><p id="par0155" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">Participated in research design&#58;</span> Xueyan Zhou&#44; Zhonghui Cao and Xiao Gao&#46;</p><p id="par0160" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">Conducted experiments&#58;</span> Zhonghui Cao&#44; Xiao Gao&#44; Jing Meng&#44; Xiaoli Guo&#44; Jiahao Xu and Junchao Cui&#46;</p><p id="par0165" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">Contributed new reagents or analytic tools&#58;</span> Jing Meng&#44; Xiaoli Guo&#44; Jiahao Xu&#44; Junchao Cui&#46;</p><p id="par0170" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">Performed data analysis&#58;</span> Zhonghui Cao&#44; Xiao Gao and Xueyan Zhou&#46;</p><p id="par0175" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">Wrote or contributed to the writing of the manuscript&#58;</span> Zhonghui Cao&#44; Xiao Gao and Xueyan Zhou&#46;</p></span><span id="sec0115" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0175">Funding</span><p id="par0180" class="elsevierStylePara elsevierViewall">This study was supported by the <span class="elsevierStyleGrantSponsor" id="gs1">Natural Science Foundation of China</span> &#91;No&#46; <span class="elsevierStyleGrantNumber" refid="gs1">82173883</span>&#93;&#59; the <span class="elsevierStyleGrantSponsor" id="gs2">Science and Technology Foundation of Xuzhou</span> &#91;No&#46; <span class="elsevierStyleGrantNumber" refid="gs2">KC21010&#44; KC22469</span>&#93;&#59; the <span class="elsevierStyleGrantSponsor" id="gs3">Natural Science Foundation of the Jiangsu Higher Education Institutions of China</span> &#91;No&#46; <span class="elsevierStyleGrantNumber" refid="gs3">18KA350002</span>&#93;&#59; the <span class="elsevierStyleGrantSponsor" id="gs4">Natural Science Foundation of Jiangsu Province</span> &#91;No&#46; <span class="elsevierStyleGrantNumber" refid="gs4">BK20181470</span>&#93;&#59; the <span class="elsevierStyleGrantSponsor" id="gs5">Provincial Commission of Health and Family Planning in Jiangsu Province</span> &#91;No&#46; <span class="elsevierStyleGrantNumber" refid="gs5">H2017079</span>&#93; and the <span class="elsevierStyleGrantSponsor" id="gs6">Science and Technology Planning Project of Jiangsu Province</span> &#91;No&#46; <span class="elsevierStyleGrantNumber" refid="gs6">BE2019636</span>&#93;&#46;</p></span><span id="sec0120" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0180">Conflict of interests</span><p id="par0185" class="elsevierStylePara elsevierViewall">The authors declare that they have no competing interests&#46;</p></span></span>"
    "textoCompletoSecciones" => array:1 [
      "secciones" => array:15 [
        0 => array:3 [
          "identificador" => "xres2093829"
          "titulo" => "Abstract"
          "secciones" => array:4 [
            0 => array:2 [
              "identificador" => "abst0005"
              "titulo" => "Background"
            ]
            1 => array:2 [
              "identificador" => "abst0010"
              "titulo" => "Methods"
            ]
            2 => array:2 [
              "identificador" => "abst0015"
              "titulo" => "Results"
            ]
            3 => array:2 [
              "identificador" => "abst0020"
              "titulo" => "Conclusions"
            ]
          ]
        ]
        1 => array:2 [
          "identificador" => "xpalclavsec1784624"
          "titulo" => "Keywords"
        ]
        2 => array:3 [
          "identificador" => "xres2093828"
          "titulo" => "Resumen"
          "secciones" => array:4 [
            0 => array:2 [
              "identificador" => "abst0025"
              "titulo" => "Antecedentes"
            ]
            1 => array:2 [
              "identificador" => "abst0030"
              "titulo" => "M&#233;todos"
            ]
            2 => array:2 [
              "identificador" => "abst0035"
              "titulo" => "Resultados"
            ]
            3 => array:2 [
              "identificador" => "abst0040"
              "titulo" => "Conclusiones"
            ]
          ]
        ]
        3 => array:2 [
          "identificador" => "xpalclavsec1784625"
          "titulo" => "Palabras clave"
        ]
        4 => array:2 [
          "identificador" => "sec0005"
          "titulo" => "Introduction"
        ]
        5 => array:3 [
          "identificador" => "sec0010"
          "titulo" => "Experimental procedure"
          "secciones" => array:10 [
            0 => array:2 [
              "identificador" => "sec0015"
              "titulo" => "Clinical studies"
            ]
            1 => array:2 [
              "identificador" => "sec0020"
              "titulo" => "Urine samples"
            ]
            2 => array:2 [
              "identificador" => "sec0025"
              "titulo" => "ELISA"
            ]
            3 => array:2 [
              "identificador" => "sec0030"
              "titulo" => "Animals"
            ]
            4 => array:2 [
              "identificador" => "sec0035"
              "titulo" => "Detection of renal function and biochemical parameters"
            ]
            5 => array:2 [
              "identificador" => "sec0040"
              "titulo" => "Cell culture and transfection"
            ]
            6 => array:2 [
              "identificador" => "sec0045"
              "titulo" => "Histopathological analysis"
            ]
            7 => array:2 [
              "identificador" => "sec0050"
              "titulo" => "Histology and immunohistochemistry analysis"
            ]
            8 => array:2 [
              "identificador" => "sec0055"
              "titulo" => "Western blot analyses"
            ]
            9 => array:2 [
              "identificador" => "sec0060"
              "titulo" => "Statistical analysis"
            ]
          ]
        ]
        6 => array:3 [
          "identificador" => "sec0065"
          "titulo" => "Results"
          "secciones" => array:5 [
            0 => array:2 [
              "identificador" => "sec0070"
              "titulo" => "Basic clinical data and biochemical indicators"
            ]
            1 => array:2 [
              "identificador" => "sec0075"
              "titulo" => "ACSL1 is overexpressed and closely related to the clinical renal function index of DKD patients"
            ]
            2 => array:2 [
              "identificador" => "sec0080"
              "titulo" => "ACSL1 expression is increased in db&#47;db mice"
            ]
            3 => array:2 [
              "identificador" => "sec0085"
              "titulo" => "The expression of ACSL1 is regulated by HG in HK-2 cells and promotes renal fibrosis"
            ]
            4 => array:2 [
              "identificador" => "sec0090"
              "titulo" => "ACSL1 knockdown reverses renal fibrosis in HG-induced HK-2 cells"
            ]
          ]
        ]
        7 => array:2 [
          "identificador" => "sec0095"
          "titulo" => "Discussion"
        ]
        8 => array:2 [
          "identificador" => "sec0100"
          "titulo" => "Conclusion"
        ]
        9 => array:2 [
          "identificador" => "sec0105"
          "titulo" => "Availability of data and materials"
        ]
        10 => array:2 [
          "identificador" => "sec0110"
          "titulo" => "Author contributions"
        ]
        11 => array:2 [
          "identificador" => "sec0115"
          "titulo" => "Funding"
        ]
        12 => array:2 [
          "identificador" => "sec0120"
          "titulo" => "Conflict of interests"
        ]
        13 => array:2 [
          "identificador" => "xack729349"
          "titulo" => "Acknowledgments"
        ]
        14 => array:1 [
          "titulo" => "References"
        ]
      ]
    ]
    "pdfFichero" => "main.pdf"
    "tienePdf" => true
    "fechaRecibido" => "2022-10-07"
    "fechaAceptado" => "2023-02-20"
    "PalabrasClave" => array:2 [
      "en" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Keywords"
          "identificador" => "xpalclavsec1784624"
          "palabras" => array:3 [
            0 => "ACSL1"
            1 => "Diabetic nephropathy"
            2 => "Renal fibrosis"
          ]
        ]
      ]
      "es" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Palabras clave"
          "identificador" => "xpalclavsec1784625"
          "palabras" => array:3 [
            0 => "ACSL1"
            1 => "Nefropat&#237;a diab&#233;tica"
            2 => "Fibrosis renal"
          ]
        ]
      ]
    ]
    "tieneResumen" => true
    "resumen" => array:2 [
      "en" => array:3 [
        "titulo" => "Abstract"
        "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Background</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Renal fibrosis is the main cause of the development of diabetic kidney disease &#40;DKD&#41;&#46; ACSL1 plays an important role in colon cancer and liver fibrosis&#46;</p></span> <span id="abst0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Methods</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">We screened ACSL1 by proteomics analysis and then verified the expression of ACSL1 in the urine of diabetic nephropathy patients by WB and ELISA&#46; Then&#44; a total of 12<span class="elsevierStyleHsp" style=""></span>db&#47;m and db&#47;db mice were used to verify the association between renal fibrosis and ACSL1&#46; Periodic acid-Schiff &#40;PAS&#41; staining&#44; Masson staining&#44; and immunostaining were performed for histological studies&#46; The relationship between ACSL1 and renal fibrosis was studied by knocking down ACSL1 in cell experiments&#46;</p></span> <span id="abst0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Results</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">The expression of ACSL1 was significantly increased in the exfoliated urine cells and urine supernatant of diabetic nephropathy patients and was closely related to renal function&#46; In addition&#44; the expression of ACSL1 was significantly increased in the renal tissues of db&#47;db mice with fibrosis&#46; Knocking down ACSL1 in HK-2 cells was shown to reverse renal fibrosis induced by high glucose&#46;</p></span> <span id="abst0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Conclusions</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">We found a potential therapeutic target for preventing or ameliorating the progression of DKD fibrosis&#46; Reducing ACSL1 expression may be a new strategy for the treatment of renal fibrosis caused by DKD&#44; which provides an experimental theoretical basis for new drug research&#46;</p></span>"
        "secciones" => array:4 [
          0 => array:2 [
            "identificador" => "abst0005"
            "titulo" => "Background"
          ]
          1 => array:2 [
            "identificador" => "abst0010"
            "titulo" => "Methods"
          ]
          2 => array:2 [
            "identificador" => "abst0015"
            "titulo" => "Results"
          ]
          3 => array:2 [
            "identificador" => "abst0020"
            "titulo" => "Conclusions"
          ]
        ]
      ]
      "es" => array:3 [
        "titulo" => "Resumen"
        "resumen" => "<span id="abst0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Antecedentes</span><p id="spar0080" class="elsevierStyleSimplePara elsevierViewall">La fibrosis renal es la causa principal de desarrollo de nefropat&#237;a diab&#233;tica &#40;ND&#41;&#46; ACSL1 juega un papel importante en el c&#225;ncer de colon y la fibrosis hep&#225;tica&#46;</p></span> <span id="abst0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">M&#233;todos</span><p id="spar0085" class="elsevierStyleSimplePara elsevierViewall">Cribamos ACSL1 mediante an&#225;lisis prote&#243;mico&#44; verificando seguidamente la expresi&#243;n de ACSL1 en la orina de los pacientes con nefropat&#237;a diab&#233;tica mediante WB y ELISA&#46; A continuaci&#243;n&#44; utilizamos un total de 12 ratones db&#47;m y db&#47;db para verificar la asociaci&#243;n entre fibrosis renal y ACSL1&#46; Se realizaron tinciones PAS &#40;Periodic acid-Schiff&#41;&#44; de Masson e inmunotinci&#243;n para los estudios histol&#243;gicos&#46; Estudiamos la relaci&#243;n entre ACSL1 y fibrosis renal aplicando la t&#233;cnica de knockdown a ACSL1 en los experimentos celulares&#46;</p></span> <span id="abst0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Resultados</span><p id="spar0090" class="elsevierStyleSimplePara elsevierViewall">La expresi&#243;n de ACSL1 se increment&#243; significativamente en las c&#233;lulas de orina exfoliada y el sobrenadante de orina de los pacientes con nefropat&#237;a diab&#233;tica y estuvo estrechamente relacionada con la funci&#243;n renal&#46; Adem&#225;s&#44; la expresi&#243;n de ACSL1 se increment&#243; significativamente en los tejidos renales de los ratones db&#47;db con fibrosis&#46; La realizaci&#243;n de knockdown a ACSL1 en las c&#233;lulas HK-2 reflej&#243; una reversi&#243;n de la fibrosis renal inducida por la alta tasa de glucosa&#46;</p></span> <span id="abst0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Conclusiones</span><p id="spar0095" class="elsevierStyleSimplePara elsevierViewall">Encontramos un objetivo terap&#233;utico potencial para prevenir o mejorar la progresi&#243;n de la fibrosis en la ND&#46; Reducir la expresi&#243;n de ACSL1 puede suponer una nueva estrategia para el tratamiento de la fibrosis renal causada por la ND&#44; lo cual aporta una base te&#243;rica experimental para la investigaci&#243;n de un nuevo f&#225;rmaco&#46;</p></span>"
        "secciones" => array:4 [
          0 => array:2 [
            "identificador" => "abst0025"
            "titulo" => "Antecedentes"
          ]
          1 => array:2 [
            "identificador" => "abst0030"
            "titulo" => "M&#233;todos"
          ]
          2 => array:2 [
            "identificador" => "abst0035"
            "titulo" => "Resultados"
          ]
          3 => array:2 [
            "identificador" => "abst0040"
            "titulo" => "Conclusiones"
          ]
        ]
      ]
    ]
    "NotaPie" => array:1 [
      0 => array:3 [
        "etiqueta" => "1"
        "nota" => "<p class="elsevierStyleNotepara" id="npar0005">These authors contributed equally to this work&#46;</p>"
        "identificador" => "fn0005"
      ]
    ]
    "multimedia" => array:7 [
      0 => array:7 [
        "identificador" => "fig0005"
        "etiqueta" => "Fig&#46; 1"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr1.jpeg"
            "Alto" => 1688
            "Ancho" => 3508
            "Tamanyo" => 273049
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">The expression of ACSL1 is increased in the urine of DKD patients&#44; and there is a correlation with renal function in DKD patients&#46; &#40;A&#41; WB analysis demonstrated that the expression level of ACSL1 was significantly increased in the urinary exfoliated cells of DKD patients&#46; &#40;B&#41; ELISA showed that the expression level of ACSL1 was significantly increased in the urine supernatant of DKD patients&#46; &#40;C&#41; Spearman correlation analysis was used to detect the correlation between urine supernatant ACSL1 and UACR&#46; &#40;D&#41; Spearman correlation analysis was used to detect the correlation between urine supernatant ACSL1 and IGU&#46; &#40;E&#41; Spearman correlation analysis was used to detect the correlation between urine supernatant ACSL1 and serum cystatin C&#46; &#40;F&#41; Spearman correlation analysis was used to detect the correlation between urine supernatant ACSL1 and eGFR&#46; &#42;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#44; compared with NC&#59; <span class="elsevierStyleSup">&#35;</span><span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#44; compared with DM patients&#46; The expression of ACSL1 in urine supernatants from NC &#40;20 healthy person&#41;&#44; DM &#40;30 diabetes mellitus&#41; and DKD &#40;34 diabetic kidney disease&#41; by ELISA&#46;</p>"
        ]
      ]
      1 => array:7 [
        "identificador" => "fig0010"
        "etiqueta" => "Fig&#46; 2"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr2.jpeg"
            "Alto" => 1310
            "Ancho" => 3500
            "Tamanyo" => 707978
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">ACSL1 expression was increased in db&#47;db mouse models&#46; &#40;A&#41; Periodic acid-Schiff staining of the renal cortex sections of db&#47;m and db&#47;db mice&#46; &#40;B&#41; Masson staining of the renal cortex sections of db&#47;m and db&#47;db mice&#46; &#40;C&#41; WB results demonstrated the expression levels of total ACSL1 in mice&#46; &#40;D&#41; Quantitation of the arbitrary ratios of ACSL1 to GAPDH&#46; &#40;E&#41; The expression of ACSL1 in mice was assessed by immunohistochemistry&#46; Data information&#58; all values are shown as the means<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>SEMs of 6&#8211;8 rats&#46; &#42;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#44; mean db&#47;db mice compared with db&#47;m mice&#46;</p>"
        ]
      ]
      2 => array:7 [
        "identificador" => "fig0015"
        "etiqueta" => "Fig&#46; 3"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr3.jpeg"
            "Alto" => 2031
            "Ancho" => 3175
            "Tamanyo" => 289345
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">Effect of HG on ACSL1 protein levels and EMT-related proteins&#46; &#40;A&#41; WB analysis demonstrated the expression levels of ACSL1 in HK-2 cells under normal glucose &#40;NG&#41;&#44; high glucose &#40;HG&#41; or mannitol &#40;MA&#41; treatment for 72<span class="elsevierStyleHsp" style=""></span>h&#46; &#40;B&#41; Quantitation of the arbitrary ratios of ACSL1 to GAPDH&#46; &#40;C&#41; WB analysis demonstrated the expression levels of EMT-related proteins in HK-2 cells under normal glucose &#40;NG&#41;&#44; high glucose &#40;HG&#41; or mannitol &#40;MA&#41; conditions for 72<span class="elsevierStyleHsp" style=""></span>h&#46; &#40;D&#41; Quantitation of the arbitrary ratios of EMT-related proteins to GAPDH&#46; Data information&#58; all values are shown as the means<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>SEMs of at least three independent experiments&#46; &#42;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#59; &#42;&#42;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;01&#44; mean HG compared with NG&#46;</p>"
        ]
      ]
      3 => array:7 [
        "identificador" => "fig0020"
        "etiqueta" => "Fig&#46; 4"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr4.jpeg"
            "Alto" => 2081
            "Ancho" => 3258
            "Tamanyo" => 302054
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">Effects of ACSL1 knockdown on renal fibrosis and EMT in HK-2 cells&#46; &#40;A&#41; WB analysis demonstrated the expression levels of ACSL1 in HK-2 cells&#46; &#40;B&#41; Quantitation of the arbitrary ratios of ACSL1 to GAPDH&#46; &#40;C&#41; WB analysis demonstrated the expression levels of EMT-related proteins in HK-2 cells&#46; &#40;D&#41; Quantitation of the arbitrary ratios of EMT-related proteins to GAPDH&#46; HG&#58; cells treated with 60<span class="elsevierStyleHsp" style=""></span>mmol&#47;L glucose&#59; HG<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>NC&#58; cells treated with HG and transfected with NC lentivirus&#59; and HG<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>ACSL1-shRNA&#58; cells treated with HG and transfected with ACSL1-shRNA lentivirus&#46; Data information&#58; all values are shown as the means<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>SEMs of at least three independent experiments&#46; &#42;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#44; &#42;&#42;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;01&#44; mean HG<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>ACSL1-shRNA compared with HG&#46;</p>"
        ]
      ]
      4 => array:8 [
        "identificador" => "tbl0005"
        "etiqueta" => "Table 1"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "detalles" => array:1 [
          0 => array:3 [
            "identificador" => "at1"
            "detalle" => "Table "
            "rol" => "short"
          ]
        ]
        "tabla" => array:2 [
          "leyenda" => "<p id="spar0050" class="elsevierStyleSimplePara elsevierViewall">BMI&#58; body mass index&#59; SBP&#58; systolic blood pressure&#59; DBP&#58; diastolic blood pressure&#59; FBG&#58; fasting blood glucose&#59; TC&#58; total cholesterol&#59; TG&#58; triglycerides&#59; HDL-C&#58; high-density lipoprotein cholesterol&#59; LDL-C&#58; low-density lipoprotein cholesterol&#59; NC&#58; nitrocellulose&#59; DM&#58; diabetes mellitus&#59; DKD&#58; diabetic kidney disease&#59; <span class="elsevierStyleSup">&#35;</span><span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#44; <span class="elsevierStyleSup">&#35;&#35;</span><span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001&#44; compared with NC&#46;</p>"
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Parameters&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t\t\t</th><th class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black">NC &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>20&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t\t\t</th><th class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black">DM &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>30&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t\t\t</th><th class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black">DKD &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>34&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Age &#40;year&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">48&#46;20<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>2&#46;76&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">51&#46;67<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>2&#46;61&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">56&#46;65<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>2&#46;19&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Gender &#40;male&#47;female&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">8&#47;12&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">20&#47;10&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">21&#47;13&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">BMI &#40;kg&#47;m<span class="elsevierStyleSup">2</span>&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">23&#46;83<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;94&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">24&#46;84<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;73&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">25&#46;85<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;67&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">SBP &#40;mmHg&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">126&#46;25<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>3&#46;26&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">127&#46;27<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>2&#46;91&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">131&#46;88<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>2&#46;73&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">DBP &#40;mmHg&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">73&#46;55<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>2&#46;49&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">81&#46;33<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>1&#46;69<span class="elsevierStyleSup">&#35;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">83&#46;09<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>1&#46;87&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">FBG &#40;mmol&#47;L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">5&#46;10<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;08&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">8&#46;45<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;52<span class="elsevierStyleSup">&#35;&#35;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">11&#46;42<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;82<span class="elsevierStyleSup">&#35;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">TC &#40;mmol&#47;L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">4&#46;85<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;18&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">4&#46;75<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;24&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">4&#46;67<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;27&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">TG &#40;mmol&#47;L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">1&#46;41<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;14&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">2&#46;01<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;37&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">2&#46;49<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;46&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">HDL-c &#40;mmol&#47;L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">1&#46;42<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;07&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">1&#46;06<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;05<span class="elsevierStyleSup">&#35;&#35;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">0&#46;98<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;04&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">LDL-c &#40;mmol&#47;L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">3&#46;15<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;15&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">3&#46;17<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;23&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">2&#46;78<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;18&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab3465227.png"
              ]
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">General data for each group&#46;</p>"
        ]
      ]
      5 => array:8 [
        "identificador" => "tbl0010"
        "etiqueta" => "Table 2"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "detalles" => array:1 [
          0 => array:3 [
            "identificador" => "at2"
            "detalle" => "Table "
            "rol" => "short"
          ]
        ]
        "tabla" => array:2 [
          "leyenda" => "<p id="spar0060" class="elsevierStyleSimplePara elsevierViewall">HbA1c&#58; hemoglobin A 1c&#59; FBG&#58; fasting blood glucose&#59; PPG&#58; postprandial blood glucose&#59; C0h&#58; C-peptide level at 0<span class="elsevierStyleHsp" style=""></span>h postprandial&#59; C1h&#58; C-Peptide level at 1<span class="elsevierStyleHsp" style=""></span>h postprandial&#59; C2h&#58; C-peptide level at 2<span class="elsevierStyleHsp" style=""></span>h postprandial&#59; INS0h&#58; insulin level at 0<span class="elsevierStyleHsp" style=""></span>h postprandial&#59; INS1h&#58; insulin level at 1<span class="elsevierStyleHsp" style=""></span>h postprandial&#59; INS2h&#58; insulin level at 2<span class="elsevierStyleHsp" style=""></span>h postprandial&#59; DM&#58; diabetes mellitus&#59; DKD&#58; diabetic kidney disease&#46;</p><p id="spar0065" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleSup">&#35;</span><span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#44; <span class="elsevierStyleSup">&#35;&#35;</span><span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;01&#44; compared with DM patients&#46;</p>"
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Parameters&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t\t\t</th><th class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black">DM &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>30&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t\t\t</th><th class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black">DKD &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>34&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">HbA1c &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">8&#46;84<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;35&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">9&#46;77<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;42&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">FBG &#40;mmol&#47;L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">8&#46;45<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;52&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">11&#46;42<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;82<span class="elsevierStyleSup">&#35;&#35;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">PPG &#40;mmol&#47;L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">15&#46;8<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;82&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">16&#46;71<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>1&#46;31&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">C0h &#40;ng&#47;mL&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">1&#46;42<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;15&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">1&#46;68<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;16&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">C1h &#40;ng&#47;mL&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">3&#46;02<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;31&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">2&#46;52<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;25&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">C2h &#40;ng&#47;mL&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">4&#46;45<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;48&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">3&#46;12<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;32<span class="elsevierStyleSup">&#35;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">INS0h &#40;pmol&#47;L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">66&#46;12<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>5&#46;64&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">198&#46;76<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>34&#46;08<span class="elsevierStyleSup">&#35;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">INS1h &#40;pmol&#47;L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">188&#46;73<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>16&#46;15&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">339&#46;89<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>58&#46;29<span class="elsevierStyleSup">&#35;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">INS2h &#40;pmol&#47;L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">215&#46;13<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>21&#46;19&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">316&#46;08<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>54&#46;21&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab3465228.png"
              ]
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0055" class="elsevierStyleSimplePara elsevierViewall">Basic clinical indicators of the DM group and DKD group&#46;</p>"
        ]
      ]
      6 => array:8 [
        "identificador" => "tbl0015"
        "etiqueta" => "Table 3"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "detalles" => array:1 [
          0 => array:3 [
            "identificador" => "at3"
            "detalle" => "Table "
            "rol" => "short"
          ]
        ]
        "tabla" => array:2 [
          "leyenda" => "<p id="spar0075" class="elsevierStyleSimplePara elsevierViewall">UCRE&#58; urine creatinine&#59; IGU&#58; urinary immunoglobulin G&#59; MA&#58; microalbumin&#59; &#945;1-M&#58; urinary &#945;1-microglobulin&#59; UACR&#58; microalbumin&#47;urine creatinine&#59; Scr&#58; serum creatinine&#59; eGFR&#58; estimated glomerular filtration rate&#59; DM&#58; diabetes mellitus&#59; DKD&#58; diabetic kidney disease&#59; <span class="elsevierStyleSup">&#35;</span><span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#44; <span class="elsevierStyleSup">&#35;&#35;</span><span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;01&#44; <span class="elsevierStyleSup">&#35;&#35;&#35;</span><span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001&#44; compared with DM patients&#46;</p>"
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Parameters&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t\t\t</th><th class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black">DM &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>30&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t\t\t</th><th class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black">DKD &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>34&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">UCRE &#40;mmol&#47;L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">10&#46;05<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;78&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">7&#46;93<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;77&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">IGU &#40;mg&#47;L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">2&#46;33<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;39&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">57&#46;32<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>22&#46;68<span class="elsevierStyleSup">&#35;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">MA &#40;mg&#47;L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">8&#46;77<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>1&#46;27&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">477&#46;12<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>219&#46;12<span class="elsevierStyleSup">&#35;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&#945;1-M &#40;mg&#47;L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">6&#46;85<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;99&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">20&#46;26<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>3&#46;26<span class="elsevierStyleSup">&#35;&#35;&#35;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">UACR &#40;mg&#47;mmol&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">0&#46;94<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;13&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">82&#46;04<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>36&#46;57<span class="elsevierStyleSup">&#35;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Serum cystatin C &#40;mg&#47;L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">0&#46;82<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;02&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">1&#46;04<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;06<span class="elsevierStyleSup">&#35;&#35;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Scr &#40;&#956;mol&#47;L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">52&#46;7<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>1&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">69&#46;44<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>6&#46;24<span class="elsevierStyleSup">&#35;&#35;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">eGFR &#40;mL&#47;&#40;min<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>1&#46;73<span class="elsevierStyleHsp" style=""></span>m<span class="elsevierStyleSup">2</span>&#41;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">126&#46;14<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>1&#46;71&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">108&#46;27<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>5&#46;07<span class="elsevierStyleSup">&#35;&#35;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab3465226.png"
              ]
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0070" class="elsevierStyleSimplePara elsevierViewall">Clinical renal function indexes of the DM group and DKD group&#46;</p>"
        ]
      ]
    ]
    "bibliografia" => array:2 [
      "titulo" => "References"
      "seccion" => array:1 [
        0 => array:2 [
          "identificador" => "bibs0015"
          "bibliografiaReferencia" => array:37 [
            0 => array:3 [
              "identificador" => "bib0190"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Update on diabetic nephropathy&#58; core curriculum 2018"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "K&#46; Umanath"
                            1 => "J&#46;B&#46; Lewis"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1053/j.ajkd.2017.10.026"
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Kidney Dis"
                        "fecha" => "2018"
                        "volumen" => "71"
                        "paginaInicial" => "884"
                        "paginaFinal" => "895"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/29398179"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            1 => array:3 [
              "identificador" => "bib0195"
              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Role of tubule epithelial cells in the pathogenesis of tubulointerstitial fibrosis induced by glomerular disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "E&#46; Healy"
                            1 => "H&#46;R&#46; Brady"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1097/00041552-199809000-00007"
                      "Revista" => array:6 [
                        "tituloSerie" => "Curr Opin Nephrol Hypertens"
                        "fecha" => "1998"
                        "volumen" => "7"
                        "paginaInicial" => "525"
                        "paginaFinal" => "530"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9818199"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "bib0200"
              "etiqueta" => "3"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Cellular and molecular mechanisms of kidney fibrosis"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "S&#46; Djudjaj"
                            1 => "P&#46; Boor"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.mam.2018.06.002"
                      "Revista" => array:6 [
                        "tituloSerie" => "Mol Aspects Med"
                        "fecha" => "2019"
                        "volumen" => "65"
                        "paginaInicial" => "16"
                        "paginaFinal" => "36"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/29909119"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            3 => array:3 [
              "identificador" => "bib0205"
              "etiqueta" => "4"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Burden of CKD&#44; proteinuria&#44; and cardiovascular risk among Chinese&#44; Mongolian&#44; and Nepalese participants in the International Society of Nephrology Screening Programs"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "S&#46;K&#46; Sharma"
                            1 => "H&#46; Zou"
                            2 => "A&#46; Togtokh"
                            3 => "B&#46; Ene-Iordache"
                            4 => "S&#46; Carminati"
                            5 => "A&#46; Remuzzi"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1053/j.ajkd.2010.06.022"
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Kidney Dis"
                        "fecha" => "2010"
                        "volumen" => "56"
                        "paginaInicial" => "915"
                        "paginaFinal" => "927"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20888105"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            4 => array:3 [
              "identificador" => "bib0210"
              "etiqueta" => "5"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Renal fibrosis&#58; novel insights into mechanisms and therapeutic targets"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "P&#46; Boor"
                            1 => "T&#46; Ostendorf"
                            2 => "J&#46; Floege"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1038/nrneph.2010.120"
                      "Revista" => array:6 [
                        "tituloSerie" => "Nat Rev Nephrol"
                        "fecha" => "2010"
                        "volumen" => "6"
                        "paginaInicial" => "643"
                        "paginaFinal" => "656"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20838416"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            5 => array:3 [
              "identificador" => "bib0215"
              "etiqueta" => "6"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Cellular and molecular mechanisms of renal fibrosis"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "Y&#46; Liu"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1038/nrneph.2011.149"
                      "Revista" => array:6 [
                        "tituloSerie" => "Nat Rev Nephrol"
                        "fecha" => "2011"
                        "volumen" => "7"
                        "paginaInicial" => "684"
                        "paginaFinal" => "696"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22009250"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            6 => array:3 [
              "identificador" => "bib0220"
              "etiqueta" => "7"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Quercetin inhibits the mTORC1&#47;p70S6K signaling-mediated renal tubular epithelial-mesenchymal transition and renal fibrosis in diabetic nephropathy"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "Q&#46; Lu"
                            1 => "X&#46; Ji"
                            2 => "Y&#46; Zhou"
                            3 => "X&#46;Q&#46; Yao"
                            4 => "Y&#46;Q&#46; Liu"
                            5 => "F&#46; Zhang"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.phrs.2015.06.006"
                      "Revista" => array:6 [
                        "tituloSerie" => "Pharmacol Res"
                        "fecha" => "2015"
                        "volumen" => "99"
                        "paginaInicial" => "237"
                        "paginaFinal" => "247"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/26151815"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            7 => array:3 [
              "identificador" => "bib0225"
              "etiqueta" => "8"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Ethanolic Ginkgo biloba leaf extract prevents renal fibrosis through Akt&#47;mTOR signaling in diabetic nephropathy"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "Q&#46; Lu"
                            1 => "W&#46;Z&#46; Zuo"
                            2 => "X&#46;J&#46; Ji"
                            3 => "Y&#46;X&#46; Zhou"
                            4 => "Y&#46;Q&#46; Liu"
                            5 => "X&#46;Q&#46; Yao"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.phymed.2015.08.010"
                      "Revista" => array:6 [
                        "tituloSerie" => "Phytomedicine"
                        "fecha" => "2015"
                        "volumen" => "22"
                        "paginaInicial" => "1071"
                        "paginaFinal" => "1078"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/26547529"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            8 => array:3 [
              "identificador" => "bib0230"
              "etiqueta" => "9"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Diabetic nephropathy&#58; an insight into molecular mechanisms and emerging therapies"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "A&#46;M&#46; Warren"
                            1 => "S&#46;T&#46; Knudsen"
                            2 => "M&#46;E&#46; Cooper"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1080/14728222.2019.1624721"
                      "Revista" => array:6 [
                        "tituloSerie" => "Expert Opin Ther Targets"
                        "fecha" => "2019"
                        "volumen" => "23"
                        "paginaInicial" => "579"
                        "paginaFinal" => "591"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/31154867"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            9 => array:3 [
              "identificador" => "bib0235"
              "etiqueta" => "10"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Diabetic nephropathy&#58; diagnosis&#44; prevention&#44; and treatment"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "J&#46;L&#46; Gross"
                            1 => "M&#46;J&#46; De Azevedo"
                            2 => "S&#46;P&#46; Silveiro"
                            3 => "L&#46;H&#46; Canani"
                            4 => "M&#46;L&#46; Caramori"
                            5 => "T&#46; Zelmanovitz"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.2337/diacare.28.1.164"
                      "Revista" => array:6 [
                        "tituloSerie" => "Diabetes Care"
                        "fecha" => "2005"
                        "volumen" => "28"
                        "paginaInicial" => "164"
                        "paginaFinal" => "176"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15616252"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            10 => array:3 [
              "identificador" => "bib0240"
              "etiqueta" => "11"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Effects of intensive glucose lowering in type 2 diabetes"
                      "autores" => array:1 [
                        0 => array:3 [
                          "colaboracion" => "Action to Control Cardiovascular Risk in Diabetes Study Group"
                          "etal" => false
                          "autores" => array:2 [
                            0 => "H&#46;C&#46; Gerstein"
                            1 => "M&#46;E&#46; Miller"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1056/NEJMoa0802743"
                      "Revista" => array:6 [
                        "tituloSerie" => "N Engl J Med"
                        "fecha" => "2008"
                        "volumen" => "358"
                        "paginaInicial" => "2545"
                        "paginaFinal" => "2559"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18539917"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            11 => array:3 [
              "identificador" => "bib0245"
              "etiqueta" => "12"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Cardiorenal end points in a trial of aliskiren for type 2 diabetes"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "H&#46;H&#46; Parving"
                            1 => "B&#46;M&#46; Brenner"
                            2 => "J&#46;J&#46; Mcmurray"
                            3 => "D&#46; de Zeeuw"
                            4 => "S&#46;M&#46; Haffner"
                            5 => "S&#46;D&#46; Solomon"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1056/NEJMoa1208799"
                      "Revista" => array:6 [
                        "tituloSerie" => "N Engl J Med"
                        "fecha" => "2012"
                        "volumen" => "367"
                        "paginaInicial" => "2204"
                        "paginaFinal" => "2213"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/23121378"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            12 => array:3 [
              "identificador" => "bib0250"
              "etiqueta" => "13"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Innate immunity in diabetic kidney disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "S&#46;C&#46;W&#46; Tang"
                            1 => "W&#46;H&#46; Yiu"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1038/s41581-019-0234-4"
                      "Revista" => array:6 [
                        "tituloSerie" => "Nat Rev Nephrol"
                        "fecha" => "2020"
                        "volumen" => "16"
                        "paginaInicial" => "206"
                        "paginaFinal" => "222"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/31942046"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            13 => array:3 [
              "identificador" => "bib0255"
              "etiqueta" => "14"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Acyl-CoA synthetases&#58; fatty acid uptake and metabolic channeling"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "M&#46; Digel"
                            1 => "R&#46; Ehehalt"
                            2 => "W&#46; Stremmel"
                            3 => "J&#46; F&#252;llekrug"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1007/s11010-008-0003-3"
                      "Revista" => array:6 [
                        "tituloSerie" => "Mol Cell Biochem"
                        "fecha" => "2009"
                        "volumen" => "326"
                        "paginaInicial" => "23"
                        "paginaFinal" => "28"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19115050"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            14 => array:3 [
              "identificador" => "bib0260"
              "etiqueta" => "15"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Fatty acid activation in carcinogenesis and cancer development&#58; essential roles of long-chain acyl-CoA synthetases"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "Y&#46; Tang"
                            1 => "J&#46; Zhou"
                            2 => "S&#46;C&#46; Hooi"
                            3 => "Y&#46;M&#46; Jiang"
                            4 => "G&#46;D&#46; Lu"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.3892/ol.2018.8843"
                      "Revista" => array:6 [
                        "tituloSerie" => "Oncol Lett"
                        "fecha" => "2018"
                        "volumen" => "16"
                        "paginaInicial" => "1390"
                        "paginaFinal" => "1396"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/30008815"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            15 => array:3 [
              "identificador" => "bib0265"
              "etiqueta" => "16"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The inhibition of Nrf2 accelerates renal lipid deposition through suppressing the ACSL1 expression in obesity-related nephropathy"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "Y&#46; Chen"
                            1 => "L&#46; He"
                            2 => "Y&#46; Yang"
                            3 => "Y&#46; Chen"
                            4 => "Y&#46; Song"
                            5 => "X&#46; Lu"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1080/0886022X.2019.1655450"
                      "Revista" => array:6 [
                        "tituloSerie" => "Ren Fail"
                        "fecha" => "2019"
                        "volumen" => "41"
                        "paginaInicial" => "821"
                        "paginaFinal" => "831"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/31488013"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            16 => array:3 [
              "identificador" => "bib0270"
              "etiqueta" => "17"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "A link between lipid metabolism and epithelial-mesenchymal transition provides a target for colon cancer therapy"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "R&#46; Sanchez-Martinez"
                            1 => "S&#46; Cruz-Gil"
                            2 => "M&#46; Gomez De Cedron"
                            3 => "M&#46; Alvarez-Fernandez"
                            4 => "T&#46; Vargas"
                            5 => "S&#46; Molina"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.18632/oncotarget.5340"
                      "Revista" => array:6 [
                        "tituloSerie" => "Oncotarget"
                        "fecha" => "2015"
                        "volumen" => "6"
                        "paginaInicial" => "38719"
                        "paginaFinal" => "38736"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/26451612"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            17 => array:3 [
              "identificador" => "bib0275"
              "etiqueta" => "18"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Insights into diabetic kidney disease using urinary proteomics and bioinformatics"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "J&#46;A&#46; Van"
                            1 => "J&#46;W&#46; Scholey"
                            2 => "A&#46; Konvalinka"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1681/ASN.2016091018"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Am Soc Nephrol"
                        "fecha" => "2017"
                        "volumen" => "28"
                        "paginaInicial" => "1050"
                        "paginaFinal" => "1061"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/28159781"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            18 => array:3 [
              "identificador" => "bib0280"
              "etiqueta" => "19"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Insights into predicting diabetic nephropathy using urinary biomarkers"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "N&#46;U&#46; Khan"
                            1 => "J&#46; Lin"
                            2 => "X&#46; Liu"
                            3 => "H&#46; Li"
                            4 => "W&#46; Lu"
                            5 => "Z&#46; Zhong"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.bbapap.2020.140475"
                      "Revista" => array:5 [
                        "tituloSerie" => "Biochim Biophys Acta Proteins Proteom"
                        "fecha" => "2020"
                        "volumen" => "1868"
                        "paginaInicial" => "140475"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/32574766"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            19 => array:3 [
              "identificador" => "bib0285"
              "etiqueta" => "20"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Urinary exosomes&#58; potential biomarkers for diabetic nephropathy"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "N&#46; Sinha"
                            1 => "V&#46; Kumar"
                            2 => "V&#46; Puri"
                            3 => "R&#46; Nada"
                            4 => "A&#46; Rastogi"
                            5 => "V&#46; Jha"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1111/nep.13720"
                      "Revista" => array:7 [
                        "tituloSerie" => "Nephrology"
                        "fecha" => "2020"
                        "volumen" => "25"
                        "paginaInicial" => "881"
                        "paginaFinal" => "887"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/32323449"
                            "web" => "Medline"
                          ]
                        ]
                        "itemHostRev" => array:3 [
                          "pii" => "S147020451830010X"
                          "estado" => "S300"
                          "issn" => "14702045"
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            20 => array:3 [
              "identificador" => "bib0290"
              "etiqueta" => "21"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Glycogen synthase kinase 3beta hyperactivity in urinary exfoliated cells predicts progression of diabetic kidney disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "X&#46; Liang"
                            1 => "P&#46; Wang"
                            2 => "B&#46; Chen"
                            3 => "Y&#46; Ge"
                            4 => "A&#46;Y&#46; Gong"
                            5 => "B&#46; Flickinger"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.kint.2019.08.036"
                      "Revista" => array:6 [
                        "tituloSerie" => "Kidney Int"
                        "fecha" => "2020"
                        "volumen" => "97"
                        "paginaInicial" => "175"
                        "paginaFinal" => "192"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/31791666"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            21 => array:3 [
              "identificador" => "bib0295"
              "etiqueta" => "22"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Human urine as a noninvasive source of kidney cells"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "F&#46; Oliveira Arcolino"
                            1 => "A&#46; Tort Piella"
                            2 => "E&#46; Papadimitriou"
                            3 => "B&#46; Bussolati"
                            4 => "D&#46;J&#46; Antonie"
                            5 => "P&#46; Murray"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1155/2015/362562"
                      "Revista" => array:5 [
                        "tituloSerie" => "Stem Cells Int"
                        "fecha" => "2015"
                        "volumen" => "2015"
                        "paginaInicial" => "362562"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/26089913"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            22 => array:3 [
              "identificador" => "bib0300"
              "etiqueta" => "23"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "KDIGO 2020 Clinical practice guideline for diabetes management in chronic kidney disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "colaboracion" => "Kidney Disease&#58; Improving Global Outcomes Diabetes Work Group"
                          "etal" => false
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.kint.2020.06.019"
                      "Revista" => array:6 [
                        "tituloSerie" => "Kidney Int"
                        "fecha" => "2020"
                        "volumen" => "98"
                        "paginaInicial" => "S1"
                        "paginaFinal" => "S115"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/32998798"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            23 => array:3 [
              "identificador" => "bib0305"
              "etiqueta" => "24"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "How to prevent the microvascular complications of type 2 diabetes beyond glucose control"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "W&#46;M&#46; Valencia"
                            1 => "H&#46; Florez"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1136/bmj.i6505"
                      "Revista" => array:5 [
                        "tituloSerie" => "BMJ"
                        "fecha" => "2017"
                        "volumen" => "356"
                        "paginaInicial" => "i6505"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/28096078"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            24 => array:3 [
              "identificador" => "bib0310"
              "etiqueta" => "25"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Angpt2 induces mesangial cell apoptosis through the microRNA-33-5p-SOCS5 loop in diabetic nephropathy"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "Y&#46;C&#46; Tsai"
                            1 => "P&#46;L&#46; Kuo"
                            2 => "W&#46;W&#46; Hung"
                            3 => "L&#46;Y&#46; Wu"
                            4 => "P&#46;H&#46; Wu"
                            5 => "W&#46;A&#46; Chang"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.omtn.2018.10.003"
                      "Revista" => array:6 [
                        "tituloSerie" => "Mol Ther Nucleic Acids"
                        "fecha" => "2018"
                        "volumen" => "13"
                        "paginaInicial" => "543"
                        "paginaFinal" => "555"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/30414568"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            25 => array:3 [
              "identificador" => "bib0315"
              "etiqueta" => "26"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Overexpression of acyl-CoA synthetase-1 increases lipid deposition in hepatic &#40;HepG2&#41; cells and rodent liver in vivo"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "H&#46;A&#46; Parkes"
                            1 => "E&#46; Preston"
                            2 => "D&#46; Wilks"
                            3 => "M&#46; Ballesteros"
                            4 => "L&#46; Carpenter"
                            5 => "L&#46; Wood"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1152/ajpendo.00112.2006"
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Physiol Endocrinol Metab"
                        "fecha" => "2006"
                        "volumen" => "291"
                        "paginaInicial" => "E737"
                        "paginaFinal" => "E744"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16705061"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            26 => array:3 [
              "identificador" => "bib0320"
              "etiqueta" => "27"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "MicroRNA-34a promotes hepatic stellate cell activation via targeting ACSL1"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "G&#46; Yan"
                            1 => "B&#46; Li"
                            2 => "X&#46; Xin"
                            3 => "M&#46; Xu"
                            4 => "G&#46; Ji"
                            5 => "H&#46; Yu"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.12659/MSM.894000"
                      "Revista" => array:6 [
                        "tituloSerie" => "Med Sci Monit"
                        "fecha" => "2015"
                        "volumen" => "21"
                        "paginaInicial" => "3008"
                        "paginaFinal" => "3015"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/26437572"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            27 => array:3 [
              "identificador" => "bib0325"
              "etiqueta" => "28"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "MiR-34c promotes hepatic stellate cell activation and liver fibrogenesis by suppressing ACSL1 expression"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "B&#46; Li"
                            1 => "J&#46; Liu"
                            2 => "X&#46; Xin"
                            3 => "L&#46; Zhang"
                            4 => "J&#46; Zhou"
                            5 => "C&#46; Xia"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.7150/ijms.51589"
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Med Sci"
                        "fecha" => "2021"
                        "volumen" => "18"
                        "paginaInicial" => "615"
                        "paginaFinal" => "625"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/33437196"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            28 => array:3 [
              "identificador" => "bib0330"
              "etiqueta" => "29"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Epithelial-to-mesenchymal transition in diabetic nephropathy&#58; fact or fiction&#63;"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "I&#46; Loeffler"
                            1 => "G&#46; Wolf"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.3390/cells4040631"
                      "Revista" => array:6 [
                        "tituloSerie" => "Cells"
                        "fecha" => "2015"
                        "volumen" => "4"
                        "paginaInicial" => "631"
                        "paginaFinal" => "652"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/26473930"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            29 => array:3 [
              "identificador" => "bib0335"
              "etiqueta" => "30"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Epoxyeicosatrienoic acid analog decreases renal fibrosis by reducing epithelial-to-mesenchymal transition"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "M&#46; Skibba"
                            1 => "M&#46;A&#46; Hye Khan"
                            2 => "L&#46;L&#46; Kolb"
                            3 => "M&#46;M&#46; Yeboah"
                            4 => "J&#46;R&#46; Falck"
                            5 => "R&#46; Amaradhi"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.3389/fphar.2017.00406"
                      "Revista" => array:5 [
                        "tituloSerie" => "Front Pharmacol"
                        "fecha" => "2017"
                        "volumen" => "8"
                        "paginaInicial" => "406"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/28713267"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            30 => array:3 [
              "identificador" => "bib0340"
              "etiqueta" => "31"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The basics of epithelial-mesenchymal transition"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "R&#46; Kalluri"
                            1 => "R&#46;A&#46; Weinberg"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1172/JCI39104"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Invest"
                        "fecha" => "2009"
                        "volumen" => "119"
                        "paginaInicial" => "1420"
                        "paginaFinal" => "1428"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19487818"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            31 => array:3 [
              "identificador" => "bib0345"
              "etiqueta" => "32"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The role of EMT in renal fibrosis"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "R&#46;M&#46; Carew"
                            1 => "B&#46; Wang"
                            2 => "P&#46; Kantharidis"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1007/s00441-011-1227-1"
                      "Revista" => array:6 [
                        "tituloSerie" => "Cell Tissue Res"
                        "fecha" => "2012"
                        "volumen" => "347"
                        "paginaInicial" => "103"
                        "paginaFinal" => "116"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/21845400"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            32 => array:3 [
              "identificador" => "bib0350"
              "etiqueta" => "33"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Update on the mechanisms of tubular cell injury in diabetic kidney disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "J&#46; Chang"
                            1 => "J&#46; Yan"
                            2 => "X&#46; Li"
                            3 => "N&#46; Liu"
                            4 => "R&#46; Zheng"
                            5 => "Y&#46; Zhong"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.3389/fmed.2021.661076"
                      "Revista" => array:5 [
                        "tituloSerie" => "Front Med &#40;Lausanne&#41;"
                        "fecha" => "2021"
                        "volumen" => "8"
                        "paginaInicial" => "661076"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/33859992"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            33 => array:3 [
              "identificador" => "bib0355"
              "etiqueta" => "34"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Renal proximal tubular epithelial cells&#58; review of isolation&#44; characterization&#44; and culturing techniques"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "M&#46; Mihevc"
                            1 => "T&#46; Petreski"
                            2 => "U&#46; Maver"
                            3 => "S&#46; Bevc"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1007/s11033-020-05977-4"
                      "Revista" => array:6 [
                        "tituloSerie" => "Mol Biol Rep"
                        "fecha" => "2020"
                        "volumen" => "47"
                        "paginaInicial" => "9865"
                        "paginaFinal" => "9882"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/33170426"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            34 => array:3 [
              "identificador" => "bib0360"
              "etiqueta" => "35"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The role of oxidative stress in diabetic complications"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "D&#46;M&#46; Niedowicz"
                            1 => "D&#46;L&#46; Daleke"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1385/CBB:43:2:289"
                      "Revista" => array:6 [
                        "tituloSerie" => "Cell Biochem Biophys"
                        "fecha" => "2005"
                        "volumen" => "43"
                        "paginaInicial" => "289"
                        "paginaFinal" => "330"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16049352"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            35 => array:3 [
              "identificador" => "bib0365"
              "etiqueta" => "36"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Endoplasmic reticulum stress and its effects on renal tubular cells apoptosis in ischemic acute kidney injury"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "Y&#46; Xu"
                            1 => "M&#46; Guo"
                            2 => "W&#46; Jiang"
                            3 => "H&#46; Dong"
                            4 => "Y&#46; Han"
                            5 => "X&#46;F&#46; An"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.3109/0886022X.2016.1160724"
                      "Revista" => array:6 [
                        "tituloSerie" => "Ren Fail"
                        "fecha" => "2016"
                        "volumen" => "38"
                        "paginaInicial" => "831"
                        "paginaFinal" => "837"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/27001462"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            36 => array:3 [
              "identificador" => "bib0370"
              "etiqueta" => "37"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "High glucose-induced epithelial-mesenchymal transition contributes to the upregulation of fibrogenic factors in retinal pigment epithelial cells"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "D&#46; Che"
                            1 => "T&#46; Zhou"
                            2 => "Y&#46; Lan"
                            3 => "J&#46; Xie"
                            4 => "H&#46; Gong"
                            5 => "C&#46; Li"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.3892/ijmm.2016.2768"
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Mol Med"
                        "fecha" => "2016"
                        "volumen" => "38"
                        "paginaInicial" => "1815"
                        "paginaFinal" => "1822"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/27748912"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
          ]
        ]
      ]
    ]
    "agradecimientos" => array:1 [
      0 => array:4 [
        "identificador" => "xack729349"
        "titulo" => "Acknowledgments"
        "texto" => "<p id="par0190" class="elsevierStylePara elsevierViewall">We thank all volunteers in this study for their cooperation&#44; and we thank the physicians from the Department of Endocrinology&#44; the Affiliated Hospital of Xuzhou Medical University for their support&#46;</p>"
        "vista" => "all"
      ]
    ]
  ]
  "idiomaDefecto" => "en"
  "url" => "/20132514/00000043000000S2/v2_202402210544/S2013251423000834/v2_202402210544/en/main.assets"
  "Apartado" => array:4 [
    "identificador" => "42660"
    "tipo" => "SECCION"
    "en" => array:2 [
      "titulo" => "Original articles"
      "idiomaDefecto" => true
    ]
    "idiomaDefecto" => "en"
  ]
  "PDF" => "https://static.elsevier.es/multimedia/20132514/00000043000000S2/v2_202402210544/S2013251423000834/v2_202402210544/en/main.pdf?idApp=UINPBA000064&text.app=https://revistanefrologia.com/"
  "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2013251423000834?idApp=UINPBA000064"
]