Serum calcium and bone: effect of PTH, phosphate, vitamin D and uremia

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"autoresLista" => "Barton S. Levine, Mariano Rodríguez, Arnold J. Felsenfeld" "autores" => array:3 [ 0 => array:3 [ "nombre" => "Barton S." "apellidos" => "Levine" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "a" "identificador" => "affa" ] ] ] 1 => array:3 [ "nombre" => "Mariano" "apellidos" => "Rodríguez" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "b" "identificador" => "affb" ] ] ] 2 => array:4 [ "nombre" => "Arnold J." "apellidos" => "Felsenfeld" "email" => array:1 [ 0 => "arnold.felsenfeld@va.gov" ] "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "a" "identificador" => "affa" ] ] ] ] "afiliaciones" => array:2 [ 0 => array:3 [ "entidad" => "Departments of Medicine, VA Greater Los Angeles Healthcare System and the David Geffen School of Medicine at UCLA, Los Angeles, CA, USA, " "etiqueta" => "a" "identificador" => "affa" ] 1 => array:3 [ "entidad" => "Department of Nephrology, Red in Ren. IMIBIC. Hospital Universitario Reina Sofía, Córdoba, Spain, " "etiqueta" => "b" "identificador" => "affb" ] ] ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Calcio sérico y huesos: efectos de la hormona paratiroidea, del fosfato, de la vitamina D y de la uremia" ] ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:8 [ "identificador" => "fig1" "etiqueta" => "Tab. 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" "figura" => array:1 [ 0 => array:4 [ "imagen" => "12379_19904_53551_en_12379_t1.jpg" "Alto" => 423 "Ancho" => 422 "Tamanyo" => 200379 ] ] "descripcion" => array:1 [ "en" => "Parathyroid hormone and calcium efflux from bone" ] ] ] "textoCompleto" => "The decreased calcemic response to parathyroid hormone (PTH) in chronic kidney disease (CKD)1,2 challenges us to evaluate how calcium is mobilized from bone. Hyperparathyroidism develops in CKD to prevent hypocalcemia. Factors contributing to the need for increased PTH include: 1) hyperphosphatemia or even inappropriately normal serum phosphorus for an elevated PTH; 2) decreased serum calcitriol; 3) downregulation of the PTH1 receptor; 4) accumulation of large, truncated amino-terminal PTH fragments such as 7-84 PTH; and 5) uremic toxins. A review of how these factors affect the calcemic response to PTH in CKD is instructive. PARATHYROID HORMONE  The relationship between PTH and serum calcium is a sigmoidal curve with basal PTH approximately 25% of the maximal PTH response to hypocalcemia.3 During hypocalcemia, increased PTH secretion restores serum calcium to normal. Besides its bone effect, PTH increases renal calcium reabsorption and calcitriol production enhancing gut absorption of calcium. However, the instantaneous effect and that of greatest magnitude is attributed to calcium efflux from bone.Not often discussed is the role of a calcium gradient with greater mobilization of calcium from bone in hypocalcemia than in hypercalcemia. When increasing PTH doses were infused in parathyroidectomized rats, the serum calcium response was curvilinear with a steeper response between 5 and 10mg/dL than at higher values (Figure 1 A) showing that the calcium gradient in hypercalcemia reduced the calcemic action of PTH.4 The effect of infused PTH on serum phosphorus was also curvilinear with a steeper decrease at lower PTH doses (Figure 1 B). These results show an important role for a calcium gradient in mobilizing calcium from bone with a possible complementary effect for phosphorus lowering.Interestingly, even in the absence of PTH, calcium efflux from bone does occur in response to reductions in serum calcium. In parathyroidectomized, hypocalcemic rats, the rate of recovery from ethylene glycol tetraacetic acid (EGTA) induced lowering of serum calcium to baseline hypocalcemic values was similar to that in parathyroid-intact rats EGTA-induced hypocalcemia to normocalcemia.5 In dogs, recovery from hypocalcemia was shown to be more rapid in young than in adult dogs, probably due to a greater exchangeable pool of bone calcium in young animals.6 Results from animal studies suggest that the increase in calcium is bone derived.5,7,8What is the short-term role of PTH and does PTH play a role in maintaining the constancy of serum calcium throughout the day? Clinical studies have shown that daily variations in serum calcium values are greater in hypoparathyroid patients than in normal humans and are affected more by variations in dietary calcium and phosphorus.9 A possible explanation is that during fasting, the normal human is better able to extract calcium from bone and during feeding to better deposit calcium into bone to maintain a constant serum calcium concentration.10,11In the early 1990s, D’Amour and associates showed that during PTH stimulation from hypocalcemia, the ratio of intact PTH to carboxy (C)-PTH fragments increased and during PTH suppression from hypercalcemia, the ratio of intact PTH to C-PTH fragments decreased.12 Subsequently, the same authors showed that besides 1-84 PTH, the assay for intact PTH also detected non 1-84 PTH large truncated amino-terminal fragments of which 7-84 PTH was the prototype.13 In normal humans, non 1-84 PTH large truncated amino-terminal fragments represent about 20% of measured intact PTH, but in renal failure, these same fragments account for approximately 50%.12,13 Interest increased when the 7-84 PTH fragment was shown to inhibit the calcemic action of 1-84 and 1-34 PTH.8,14 The action of 7-84 PTH is most likely mediated by its binding to the C-PTH receptor present on osteocytes and other cells of osteoblast lineage including lining cells on the bone surface.15,16 Moreover, the osteocyte has important signaling functions to the bone surface.17 Finally, 7-84 PTH may internalize the PTH1 receptor in selected target cells in kidney and bone18 and also reduce calcitriol production.19 Thus, in advanced renal failure, greatly increased 7-84 PTH could decrease the calcemic response to PTH in absence of hypercalcemia.2An intriguing observation in azotemic rats is that parathyroidectomy completely restored the calcemic response to PTH even though hyperphosphatemia was present before the 1-34 PTH infusion (Figure 2 A).20 Before the PTH infusion, rats were maintained on a high calcium diet for more than two weeks after parathyroidectomy. It is possible that the total absence of 7-84 PTH or other PTH fragments resulted in the greater response to PTH. Other possibilities not mutually exclusive include that the total absence of PTH resulted in an upregulation of the PTH1 receptor, hypocalcemia lessened the gradient driving mineralization increasing the exchangeable bone pool of calcium, and the high calcium diet resulted in expansion of the exchangeable bone pool of calcium.The size of the exchangeable pool of bone calcium differs among the different forms of renal osteodystrophy in CKD. In studies in hemodialysis patients performed in the 1980s and 1990s, we used low and high calcium dialysates during hemodialysis to induce hypo- and hypercalcemia to evaluate PTH stimulation and suppression. The capacity to defend against the development of hypocalcemia and hypercalcemia was much greater in hemodialysis patients with high (osteitis fibrosa) than with low bone turnover (adynamic bone or osteomalacia).21-23 A similarly expanded pool of bone calcium and increased calcium efflux from bone has been shown in dialysis patients with osteitis fibrosa studied with calcium isotopes.24 Moreover, in osteitis fibrosa, bone is deposited in a woven rather than the usual lamellar pattern. The abnormal woven bone may bind or incorporate calcium less well.25 Also, trabecular bone volume and thickness were greater in dialysis patients with high than low bone turnover suggesting that greater bone mass is present for calcium exchange in the former.25 A summary of the discussion on PTH and bone is provided in Table 1. PHOSPHATE  Phosphate is an important factor in evaluating the calcemic response to PTH. Long ago Albright showed that the first action of infused parathyroid extract was phosphaturia followed by lowering serum phosphorus before a calcemic effect developed.26 In the 1960s, Raisz showed in an ex-vivo study that increasing the phosphate concentration in the medium reduced calcium efflux from bone both in the absence and presence of PTH.27 Phosphate also modifies calcium efflux from bone in animal and human studies.28-30 In parathyroidectomized rats given a normal phosphate diet and a fixed replacement dose of PTH sufficient to maintain normal serum calcium and phosphorus values, changing to a high phosphate diet resulted in hypocalcemia and hyperphosphatemia.29 In young, healthy subjects given continuous intravenous phosphate infusion for 7 days, hypocalcemia and hyperphosphatemia developed until a three-fold increase in PTH increased phosphate excretion by five-fold and normalized serum calcium and phosphorus.28 Conversely, phosphate depletion induces hypercalcemia despite marked suppression of PTH.31 Even high dose bisphosphonate in phosphate-depleted rats did not prevent hypercalcemia despite producing toxic effects in osteoclasts and a marked decrease in the osteoblast surface.31 These results support the concept that an osteoclast independent effect may be important for bone efflux of calcium.32,33 Finally, bisphosphonate treatment in phosphate-depleted rats increased urine calcium excretion more than in phosphate-depleted rats not receiving bisphosphonates suggesting that decreased calcium influx to bone may be a factor.In renal failure, many studies have shown that phosphate loading or hyperphosphatemia decreases the calcemic response to PTH.34-36 When 5/6 nephrectomy was performed in parathyroidectomized rats, a replacement dose of 1-34 PTH sufficient to maintain normal serum calcium and phosphorus values in parathyroidectomized rats with normal renal function failed to maintain normal serum calcium and phosphorus levels in renal failure.29 Even though dietary phosphate was unchanged, the phosphate burden was presumably increased because of decreased renal excretion of phosphate.29 Conversely, dietary phosphate restriction in renal failure decreases PTH while maintaining the serum calcium value. In essence, less PTH is needed to maintain the serum calcium concentration.37-40 Even in patients with advanced CKD, dietary phosphate restriction decreases PTH while maintaining normal serum calcium values.41,42 A summary of the discussion of the effect of phosphate on serum calcium and bone is provided in Table 2. VITAMIN D  The effect of vitamin D on the exchangeable pool of bone calcium is more difficult to define than that of phosphate. Under some circumstances, 1,25 vitamin D (1,25D) appears to enhance, but in others to reduce calcium efflux from bone. In vitamin D intoxication, studies have suggested that calcium efflux from bone is increased.43-48 In vitro and in vivo studies have shown that calcitriol increases osteoclast activity.49-51 In a case report of vitamin D intoxication, bone histomorphometry showed an increase in osteoclasts with a reduction in osteoblasts, an increased osteoid surface and volume, and decreased mineralization.45 Studies in the chick also found that vitamin D intoxication impaired bone formation.52,53 The effects on osteoid volume and bone formation suggest that calcium influx to bone may be decreased in vitamin D intoxication. Both corticosteroids and bisphosphonates effectively treat hypercalcemia in vitamin D intoxication.43-45 However, in different settings, bisphosphonate treatment did not change the rate of recovery from EDTA-induced hypocalcemia in osteoporotic patients54 or prevent hypercalcemia in rats on a phosphate depleted diet perhaps suggesting different mechanisms.31In a recent study in intestinal-specific VDR-null mice in which calcium absorption is unresponsive to 1,25D, insights into the calcemic and bone effects of 1,25D during calcium deficiency were provided.55 In this mouse model, markedly elevated endogenous 1,25D levels maintained serum calcium normal by increasing calcium efflux from bone while bone mineralization was inhibited resulting in hyperosteoidosis and a decrease in bone mass. The activation of bone resorption by 1,25D in calcium deficient intestinal-specific VDR-null mice was osteoblast mediated. In systemic VDR-receptor null mice, bone abnormalities were prevented when calcium repletion was achieved with a high calcium/lactose diet.56 In the intestinal-specific null model, although PTH values were elevated, the authors concluded that because markedly elevated PTH did not prevent hypocalcemia in systemic VDR null mice, 1,25D played a critical role in increasing calcium efflux from bone in intestinal-specific VDR-null mice. Finally, the calcemic bone actions of 1,25D were blocked by bisphosphonates. The authors concluded that high 1,25D levels in calcium deficiency: 1) increase calcium efflux from bone; 2) decrease calcium influx to bone by inhibiting bone mineralization; and 3) both 1) and 2) act to maintain serum calcium, but decrease bone mass.In intestinal-specific VDR null mice, a marked elevation in 1,25D increased calcium efflux from bone.55 But a major question is whether such a mechanism is applicable in vitamin D deficiency? In dogs placed on a vitamin D and calcium deficient diet, 25D values decreased rapidly, but 1,25D values tripled by 24 weeks and remained greater than normal at 36 weeks before subsequently declining below normal.57 Ionized calcium values remained normal until 24 weeks before marginally decreasing at 36 weeks. Thus, despite vitamin D and calcium deficiency, serum calcium values were maintained normal for a prolonged time as 1,25D and PTH values tripled during the first 24 weeks. An elevated 1,25D and PTH together with hypophosphatemia contributed to the prolonged maintenance of serum calcium. The marked 1,25D increase during the first 24 weeks could have helped maintain serum calcium by enhancing calcium efflux from bone while also decreasing calcium influx to bone. In humans with established vitamin D deficiency, studies have shown that treatment with vitamin D or 25D increases 1,25D values to 3 to 4 times greater than normal.58-60 Thus, it is plausible in vitamin D deficiency that high 1,25D values have an important skeletal effect in maintaining serum calcium during its evolution and increasing serum calcium during initial treatment with vitamin D. Also, in evolving vitamin D deficiency, high 1,25D values perhaps together with PTH-induced decreases in serum phosphorus potentially contribute to the development of osteomalacia.In renal failure, several studies have shown that acute calcitriol administration increases the calcemic response to PTH.61,62 In one study, high dose PTH was infused for 48 hours while calcitriol was administered to rats on a calcium free diet.20 Thus, neither calcitriol nor hypercalcemia could modify PTH levels. Calcitriol administration enhanced the calcemic response to PTH in azotemic rats on a low phosphate diet and in both normal and azotemic rats on a high phosphate diet (Figure 2 B).20Two studies in dialysis patients are of particular interest because long-term calcitriol treatment decreased the bone response to PTH.63,64 In one study, 14 children on peritoneal dialysis with biopsy-proven osteitis fibrosa were treated with high dose calcitriol for 12 months with dose adjustments for hypercalcemia and hyperphosphatemia.63 The average calcitriol dose was 1.25µg thrice weekly. The dialysate calcium concentration was 3.5meq/L and calcium carbonate was the primary phosphate binder. A repeat bone biopsy performed after one year showed that 12 patients developed normal or adynamic bone and in 6 patients the bone formation rate decreased despite no change in PTH values. The latter result suggests that calcitriol treatment may have decreased the bone formation rate similar to results seen in intestinal-specific VDR null mice.55 A positive calcium balance from calcium carbonate and high dialysate calcium concentration could also have contributed.In the other study, a hemodialysis patient with markedly elevated intact PTH (1912pg/mL, Nichols assay) and serum alkaline phosphatase (1512IU/L) was studied before calcitriol treatment, after 6 weeks of 2µg intravenous calcitriol thrice weekly, and 6 weeks after calcitriol was stopped.64 Studies consisted of separate dialysis sessions with low (1meq/L) and high (4meq/L) calcium dialysate to determine maximal PTH secretion and suppression respectively. Despite 6 weeks of calcitriol treatment, predialysis serum calcium and PTH values were similar to pretreatment values (Figure 3 A and Figure 3 B) as were serum phosphorus and alkaline phosphatase values. The maximal PTH value during induction of hypocalcemia was similar approaching 4000pg/mL during both low calcium dialysis studies (Figure 3 B). However, during the low calcium dialysis in the first study, serum calcium, which started at 10.8mg/dL, only decreased to 9.4mg/dL at 180 minutes. After calcitriol treatment, despite a similar starting serum calcium concentration, serum calcium decreased to less than 8mg/dL after only 15 minutes of dialysis (Figure 3A). Studies with the high calcium dialysate showed marked differences in bone buffering of calcium. Before calcitriol treatment, serum calcium slowly increased to 11.8mg/dL at 180 minutes. However, after calcitriol treatment, serum calcium increased to 12.2mg/dL after only 15 minutes (Figure 3 C). PTH suppression was similar (Figure 3 D). When dialysis studies were repeated after calcitriol had been stopped for 6 weeks, results were similar to those before calcitriol was started (Figure 3). Thus, in this hemodialysis patient with severe secondary hyperparathyroidism and an enhanced capacity to defend against the induction of hypocalcemia and hypercalcemia, calcitriol treatment for 6 weeks dramatically decreased calcium efflux from and influx to bone.In both cited studies, a decreased bone/calcemic response to PTH was seen. In the second study, the exchangeable pool of bone calcium was seemingly greatly reduced by calcitriol treatment despite no change in PTH secretion. In the first study it could be argued that the decrease in osteoblasts and osteoclasts, which reflects bone remodeling, is different from an evaluation of bone efflux and influx of calcium. However, the marked reduction in bone formation rate together with the decreased cellular activity seen after calcitriol treatment is also a likely indicator of a decreased exchangeable pool of bone calcium.How does the vitamin D/calcium biochemical profile differ between dialysis patients receiving high doses of calcitriol and patients with vitamin D intoxication? In dialysis patients, calcitriol treatment reduced calcium efflux from bone during a low calcium dialysis while in patients with vitamin D intoxication increased calcium efflux from bone is reported to be a major contributor to hypercalcemia. In vitamin D intoxication, hypercalcemia is persistent with suppressed PTH. In dialysis patients treated with calcitriol, if monitored diligently the serum calcium concentration is normal while the high PTH value generally decreases, but remains elevated. In the study in which calcitriol was given thrice weekly, measured serum calcitriol values were in the normal range before subsequent dosing,63 but high 1,25D values would be expected immediately after calcitriol dosing.65 In dialysis patients, 25OHD values are often less than the desired goal, 30ng/mL. Conversely, in vitamin D intoxication, 25OHD values are markedly elevated while serum calcitriol values are often but not always increased.66-68How does vitamin D affect calcium efflux from bone in vitamin D intoxication? In vitamin D intoxication, longstanding hypercalcemia leads to reduced renal function further exacerbating hypercalcemia, which may act to drive calcium into bone. Treatment with normal saline improves renal function by correcting the decreased extracellular volume, which in turn, increases renal excretion of calcium lowering serum calcium. As a result, the supersaturated bone releases more calcium for several reasons.43-45 These include an increasing calcium gradient between bone and blood, reduced serum phosphorus from improved renal function, and increased PTH secretion from a reduction in serum calcium. Such actions could account for the enhanced calcium release from bone reported in vitamin D intoxication. Finally, suggesting that increased bone calcium deposition may occur during the hypercalcemia of vitamin D intoxication is the report of increased bone mineral density in osteoporotic patients with vitamin D intoxication.48Why did high dose calcitriol treatment decrease the exchangeable pool of bone calcium in the dialysis patient with severe secondary hyperparathyroidism? In dialysis patients with osteitis fibrosa, there are abundant osteoblasts in which the VDR can be activated to generate osteoclasts and increase bone resorption. However, calcitriol administration decreased the exchangeable pool of bone calcium. In both cited studies in patients with osteitis fibrosa, a calcium-replete environment was present due to high dialysate calcium, the use of calcium-based phosphate binders, and intermittent or continual hypercalcemia.63,64 In contrast, in studies in intestinal-specific VDR-null mice in which 1,25D-mediated increased bone resorption and decreased bone formation were seen, calcium depletion was present.55 However, when the VDR-null mouse was calcium replete, increased bone resorption and decreased bone formation were prevented.56 Other important differences are present between dialysis patients with osteitis fibrosa and intestinal-specific VDR null mice/patients with vitamin D deficiency. In the dialysis patients, calcitriol administration was intermittent in contrast to continuously elevated, endogenous 1,25D values. Although PTH values are increased in all the cited conditions, the calcium and phosphorus status are quite different. In the dialysis patients, hypercalcemia and hyperphosphatemia are present while in intestinal specific null mice and vitamin D deficient patients, hypocalcemia and hypophosphatemia prevail. In the reported dialysis patient,64 increases in serum calcium were not seen with calcitriol treatment. However, whether increased bone buffering of calcium not only prevented hypercalcemia, but also drove the mineralization process decreasing the exchangeable pool of bone calcium is an interesting possibility. A summary of the discussion on 1,25D and bone is provided Table 3.A final point of discussion is the effect of both 19-nor-1,25-dihydroxy-vitamin D2 (paricalcitol), an analog of 1,25D, and the calcimimetic, cinacalcet, on bone resorption and serum calcium. Both agents have been used for the past 10 to 15 years for the treatment of hyperparathyroidism in dialysis patients. To achieve similar PTH suppression, the dose ratio of paricalcitol to calcitriol used has been 3 or 4:1. Most, but not all in vitro and animal studies have shown that the calcemic effect of paricalcitol is less than that of calcitriol.69-71 In studies in dialysis patients, paricalcitol suppresses PTH secretion at least as well if not better than calcitriol72 and may also have less calcemic effect.72,73 In studies in uremic rats, less vascular calcification has been seen with paricalcitol than with calcitriol74 and calcimimetics appear to be even more effective in preventing vascular calcification.75 A summary of the discussion on 1,25D and bone is provided Table 3.Calcimimetics are an effective treatment for hyperparathyroidism in the uremic animals and dialysis patient. Because of its calcimimetic activity, hypocalcemia generally develops during treatment. Several studies in rats have evaluated the effect of calcimimetics on bone.76-78 Two studies in rats used a 5/6 nephrectomy model76,78 and the other an adenine model of uremia.77 The latter model results in much more severe renal failure, hyperparathyroidism, hypocalcemia and hyperphosphatemia than the former model. In one of the 5/6 nephrectomy models, a high phosphate diet was used78 and in the other a relatively high calcium diet was used.76 Moreover, different calcimimetics were used in the three studies. In all the studies, serum calcium and PTH decreased, but the magnitude of the reduction in PTH differed. Changes in bone histology also differed considerably among the studies. In another study simulating primary hyperparathyroidism, the bone response to a two week continuous infusion of PTH was evaluated in mice with either a PTH gene knockout or PTH and calcium sensing receptor gene knockout (double knockout). The mice had normal renal function. The absence of the calcium sensing receptor reduced the bone effects of PTH in this model of primary hyperparathyroidism.79 UREMIC TOXINS  A variety of factors which could potentially contribute to skeletal resistance to PTH are altered in CKD. Exposure of osteoblast-like cells to uremic serum affects the number and function of these cells including suppressed mitogenesis and IL-6 release and reduced PTHR expression.80-82 In CKD, levels of inhibitory and stimulatory insulin growth factor binding proteins 4 and 5 respectively, are altered and may contribute to abnormal osteoblast proliferation.80 Osteoprotegerin, a decoy receptor of NF-kB ligand (RANKL), accumulates as renal failure progresses blocking osteoclast activation by PTH.The concentration of absorbed products of intestinal microbial flora increase as renal function declines. These products, particularly indoxyl sulfate and p-cresol sulfate, may alter bone metabolism and contribute to bone resistance to PTH. Indole, formed by gastrointestinal bacteria during breakdown of tryptophan, is metabolized to indoxyl sulfate which is transported by an organic anion transporter into osteoblasts where it induces oxidative stress. Exposure of osteoblast cells in culture to indoxyl sulfate inhibits PTH-induced cyclic AMP generation and downregulates PTHR expression, an effect partially inhibited by the organic anion transporter inhibitor, probenicid.83 Elevated indoxyl sulfate values in hemodialysis patients inversely correlate with markers of bone formation.84 Furthermore, in a rat model of adynamic bone in which elevated indoxyl sulfate values were present, administration of the oral absorbent, AST-120, lowered indoxyl sulfate values and increased bone formation.85Indoxyl sulfate and p-cresol sulfate, a gut microbial product produced from metabolism of tyrosine, may indirectly alter PTH action on bone through the FGF23-klotho axis. Klotho blood values are low in CKD while FGF23 values are markedly elevated. In a mouse model of CKD and in isolated kidney cells, both indoxyl sulfate and p-cresol sulfate suppressed klotho production by epigenetic modification of the klotho gene.86 These toxins stimulate expression of DNA methyltransferase 1 which hypermethylates the klotho gene suppressing gene expression. A deficiency of klotho as exemplified by the klotho knockout mouse results in elevated values of serum calcium, phosphorus and 1,25D, ectopic calcification including extensive vascular calcification, and osteoporosis with decreases in osteoblasts and osteoclasts.87 FGF23 values are markedly elevated in the klotho knockout model similar to that in CKD. Thus, reduced levels of klotho in CKD, perhaps at least in part due to uremic toxins, could alter the bone response to PTH via hyperphosphatemia and/or extremely elevated FGF23 levels found in CKD.88 The potential role of uremic toxins on bone is summarized in Table 4.A summary of the many factors affecting calcium efflux from bone discussed in our review is shown in Figure 4. Many unanswered questions exist about the exchangeable pool of bone calcium. These include 1) how PTH mobilizes calcium from bone, 2) what is the contribution of osteoclast-mediated calcium efflux in contrast to shifts across the quiescent bone surface, 3) how after induced hypocalcemia, serum calcium is restored to baseline values in parathyroidectomized animals, 4) how parathyroidectomy restores the calcemic response to PTH to normal in the azotemic rat, 5) how phosphate modifies calcium efflux from bone, 6) why bisphosphonate treatment reduces calcium efflux from bone in vitamin D intoxication, but not in phosphate depletion, 7) how calcitriol treatment decreases the exchangeable pool of bone calcium in dialysis patients with osteitis fibrosa, and 8) the role of uremic toxins on the exchangeable pool of bone calcium. Much remains to be learned about the role of bone in the regulation of serum calcium. ACKNOWLEDGMENTS M.R. has received grant support from the Instituto Carlos III (FIS 07/0287, FIS 07/0315, 010/1311), Consejería de Salud (JA 0127/2008), a UE Grant from Framework Programme 7 Syskid (FP7-241544), and Consejería de Innovación, Ciencia y Empresa (CTS-5205 and CTS-170) of Junta de Andalucía. DISCLOSURES B.L and A.F. have no disclosures. M.R. has received grants from Amgen and Fresenius and lecture fees from Amgen, Abbott, Shire and Fresenius. Conflicts of interest The authors declare that they have no conflicts of interest related to the contents of this article.<a href="grande/12379_19904_53551_en_12379_t1.jpg" class="elsevierStyleCrossRefs"><img src="12379_19904_53551_en_12379_t1.jpg" alt="Parathyroid hormone and calcium efflux from bone"></img></a>Table 1. Parathyroid hormone and calcium efflux from bone<a href="grande/12379_19904_53552_en_12379_t2.jpg" class="elsevierStyleCrossRefs"><img src="12379_19904_53552_en_12379_t2.jpg" alt="Phosphorus and calcium efflux from bone"></img></a>Table 2. Phosphorus and calcium efflux from bone<a href="grande/12379_19904_53553_en_12379_t3.jpg" class="elsevierStyleCrossRefs"><img src="12379_19904_53553_en_12379_t3.jpg" alt="Calcitriol and calcium efflux from bone"></img></a>Table 3. Calcitriol and calcium efflux from bone<a href="grande/12379_19904_53554_en_12379_t4.jpg" class="elsevierStyleCrossRefs"><img src="12379_19904_53554_en_12379_t4.jpg" alt="Uremic toxins and calcium efflux from bone"></img></a>Table 4. Uremic toxins and calcium efflux from bone<a href="grande/12379_19904_53555_en_12379_f1.jpg" class="elsevierStyleCrossRefs"><img src="12379_19904_53555_en_12379_f1.jpg" alt="Serum calcium and phosphorus response to PTH dosing in parathyroidectomized rats."></img></a>Figure 1. Serum calcium and phosphorus response to PTH dosing in parathyroidectomized rats.<a href="grande/12379_19904_53557_en_12379_f2_copy1.jpg" class="elsevierStyleCrossRefs"><img src="12379_19904_53557_en_12379_f2_copy1.jpg" alt="Changes in the serum level of calcium and phosphorus during PTH infusion for 48 hours."></img></a>Figure 2. Changes in the serum level of calcium and phosphorus during PTH infusion for 48 hours.<a href="grande/12379_19904_53558_en_12379_f3.jpg" class="elsevierStyleCrossRefs"><img src="12379_19904_53558_en_12379_f3.jpg" alt="Serum calcium and PTH response to low and high calcium dialysate before, during and after calcitriol (CTR) treatment."></img></a>Figure 3. Serum calcium and PTH response to low and high calcium dialysate before, during and after calcitriol (CTR) treatment.<a href="grande/12379_19904_53560_en_12379_f4.jpg" class="elsevierStyleCrossRefs"><img src="12379_19904_53560_en_12379_f4.jpg" alt="Schematic of factors affecting calcium efflux from bone are shown."></img></a>Figure 4. Schematic of factors affecting calcium efflux from bone are shown." "pdfFichero" => "P1-E574-S4695-A12379-EN.pdf" "tienePdf" => true "PalabrasClave" => array:2 [ "es" => array:6 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Palabras clave" "identificador" => "xpalclavsec437565" "palabras" => array:1 [ 0 => "Vitamina D" ] ] 1 => array:4 [ "clase" => "keyword" "titulo" => "Palabras clave" "identificador" => "xpalclavsec437567" "palabras" => array:1 [ 0 => "Uremia" ] ] 2 => array:4 [ "clase" => "keyword" "titulo" => "Palabras clave" "identificador" => "xpalclavsec437569" "palabras" => array:1 [ 0 => "Fosfato" ] ] 3 => array:4 [ "clase" => "keyword" "titulo" => "Palabras clave" "identificador" => "xpalclavsec437576" "palabras" => array:1 [ 0 => "Hormona paratiroidea" ] ] 4 => array:4 [ "clase" => "keyword" "titulo" => "Palabras clave" "identificador" => "xpalclavsec437580" "palabras" => array:1 [ 0 => "Calcio" ] ] 5 => array:4 [ "clase" => "keyword" "titulo" => "Palabras clave" "identificador" => "xpalclavsec437583" "palabras" => array:1 [ 0 => "Hueso" ] ] ] "en" => array:6 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec437566" "palabras" => array:1 [ 0 => "Vitamin D" ] ] 1 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec437568" "palabras" => array:1 [ 0 => "Uremia" ] ] 2 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec437572" "palabras" => array:1 [ 0 => "Phosphate" ] ] 3 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec437578" "palabras" => array:1 [ 0 => "Parathyroid hormone" ] ] 4 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec437582" "palabras" => array:1 [ 0 => "Calcium" ] ] 5 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec437584" "palabras" => array:1 [ 0 => "Bone" ] ] ] ] "tieneResumen" => true "resumen" => array:2 [ "es" => array:1 [ "resumen" => "El hiperparatiroidismo se desarrolla en la enfermedad renal crónica (ERC). La disminución de la respuesta calcémica a la hormona paratiroidea (PTH) contribuye al desarrollo de hiperparatiroidismo y es probable que se deba a una reducción de la emisión de calcio de los huesos. Entre los factores que contribuyen a la disminución de la respuesta calcémica a la PTH en la ERC se encuentran: 1) la hiperfosfatemia; 2) la disminución del calcitriol sérico; 3) la desensibilización del receptor PTHR1; 4) la presencia de fragmentos de gran tamaño de los extremos aminoterminales de la hormona paratiroidea que actúan en el receptor carboxi-PTH y 5) las toxinas urémicas. Asimismo, la administración prolongada de una dosis elevada de calcitriol podría disminuir la reserva intercambiable de calcio independiente de la hormona paratiroidea. El objetivo de esta revisión es facilitar la comprensión de cómo afectan los factores mencionados anteriormente a la emisión de calcio procedente del hueso en la ERC. Como conclusión, aún queda mucho por aprender acerca del papel de los huesos en la regulación del calcio sérico." ] "en" => array:1 [ "resumen" => "Hyperparathyroidism develops in chronic kidney disease (CKD). A decreased calcemic response to parathyroid hormone (PTH) contributes to the development of hyperparathyroidism and is presumed due to reduced calcium efflux from bone. Contributing factors to the decreased calcemic response to PTH in CKD include: 1) hyperphosphatemia; 2) decreased serum calcitriol; 3) downregulation of the PTH1 receptor; 4) large, truncated amino-terminal PTH fragments acting at the carboxy-PTH receptor; and 5) uremic toxins. Also, prolonged high dose calcitriol administration may decrease the exchangeable pool of bone calcium independent of PTH. The goal of the review is to provide a better understanding of how the above cited factors affect calcium efflux from bone in CKD. In conclusion, much remains to be learned about the role of bone in the regulation of serum calcium." ] ] "multimedia" => array:8 [ 0 => array:8 [ "identificador" => "fig1" "etiqueta" => "Tab. 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" "figura" => array:1 [ 0 => array:4 [ "imagen" => "12379_19904_53551_en_12379_t1.jpg" "Alto" => 423 "Ancho" => 422 "Tamanyo" => 200379 ] ] "descripcion" => array:1 [ "en" => "Parathyroid hormone and calcium efflux from bone" ] ] 1 => array:8 [ "identificador" => "fig2" "etiqueta" => "Tab. 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" "figura" => array:1 [ 0 => array:4 [ "imagen" => "12379_19904_53552_en_12379_t2.jpg" "Alto" => 443 "Ancho" => 423 "Tamanyo" => 234569 ] ] "descripcion" => array:1 [ "en" => "Phosphorus and calcium efflux from bone" ] ] 2 => array:8 [ "identificador" => "fig3" "etiqueta" => "Tab. 3" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" "figura" => array:1 [ 0 => array:4 [ "imagen" => "12379_19904_53553_en_12379_t3.jpg" "Alto" => 483 "Ancho" => 423 "Tamanyo" => 272648 ] ] "descripcion" => array:1 [ "en" => "Calcitriol and calcium efflux from bone" ] ] 3 => array:8 [ "identificador" => "fig4" "etiqueta" => "Tab. 4" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" "figura" => array:1 [ 0 => array:4 [ "imagen" => "12379_19904_53554_en_12379_t4.jpg" "Alto" => 430 "Ancho" => 418 "Tamanyo" => 204098 ] ] "descripcion" => array:1 [ "en" => "Uremic toxins and calcium efflux from bone" ] ] 4 => array:8 [ "identificador" => "fig5" "etiqueta" => "Fig. 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" "figura" => array:1 [ 0 => array:4 [ "imagen" => "12379_19904_53555_en_12379_f1.jpg" "Alto" => 335 "Ancho" => 701 "Tamanyo" => 137836 ] ] "descripcion" => array:1 [ "en" => "Serum calcium and phosphorus response to PTH dosing in parathyroidectomized rats." ] ] 5 => array:8 [ "identificador" => "fig6" "etiqueta" => "Fig. 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" "figura" => array:1 [ 0 => array:4 [ "imagen" => "12379_19904_53557_en_12379_f2_copy1.jpg" "Alto" => 766 "Ancho" => 701 "Tamanyo" => 217124 ] ] "descripcion" => array:1 [ "en" => "Changes in the serum level of calcium and phosphorus during PTH infusion for 48 hours." ] ] 6 => array:8 [ "identificador" => "fig7" "etiqueta" => "Fig. 3" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" "figura" => array:1 [ 0 => array:4 [ "imagen" => "12379_19904_53558_en_12379_f3.jpg" "Alto" => 557 "Ancho" => 700 "Tamanyo" => 218449 ] ] "descripcion" => array:1 [ "en" => "Serum calcium and PTH response to low and high calcium dialysate before, during and after calcitriol (CTR) treatment." ] ] 7 => array:8 [ "identificador" => "fig8" "etiqueta" => "Fig. 4" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" "figura" => array:1 [ 0 => array:4 [ "imagen" => "12379_19904_53560_en_12379_f4.jpg" "Alto" => 407 "Ancho" => 699 "Tamanyo" => 151069 ] ] "descripcion" => array:1 [ "en" => "Schematic of factors affecting calcium efflux from bone are shown." ] ] ] "bibliografia" => array:2 [ "titulo" => "Bibliography" "seccion" => array:1 [ 0 => array:1 [ "bibliografiaReferencia" => array:88 [ 0 => array:3 [ "identificador" => "bib1" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:3 [ "referenciaCompleta" => "Massry SG, Coburn JW, Lee DBW, Jowsey J, Kleeman CR. 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Calcio sérico y huesos: efectos de la hormona paratiroidea, del fosfato, de la vitamina D y de la uremia

Barton S. Levinea, Mariano Rodríguezb, Arnold J. Felsenfelda

a Departments of Medicine, VA Greater Los Angeles Healthcare System and the David Geffen School of Medicine at UCLA, Los Angeles, CA, USA,

b Department of Nephrology, Red in Ren. IMIBIC. Hospital Universitario Reina Sofía, Córdoba, Spain,

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    "textoCompleto" => "<p class="elsevierStylePara">The decreased calcemic response to parathyroid hormone &#40;PTH&#41; in chronic kidney disease &#40;CKD&#41;<span class="elsevierStyleSup">1&#44;2</span> challenges us to evaluate how calcium is mobilized from bone&#46; Hyperparathyroidism develops in CKD to prevent hypocalcemia&#46; Factors contributing to the need for increased PTH include&#58; 1&#41; hyperphosphatemia or even inappropriately normal serum phosphorus for an elevated PTH&#59; 2&#41; decreased serum calcitriol&#59; 3&#41; downregulation of the PTH1 receptor&#59; 4&#41; accumulation of large&#44; truncated amino-terminal PTH fragments such as 7-84 PTH&#59; and 5&#41; uremic toxins&#46; A review of how these factors affect the calcemic response to PTH in CKD is instructive&#46;</p><p class="elsevierStylePara"><span class="elsevierStyleBold">&#160;</span></p><p class="elsevierStylePara"><span class="elsevierStyleBold">PARATHYROID HORMONE</span><span class="elsevierStyleBold">&#160;</span></p><p class="elsevierStylePara">&#160;</p><p class="elsevierStylePara">The relationship between PTH and serum calcium is a sigmoidal curve with basal PTH approximately 25&#37; of the maximal PTH response to hypocalcemia&#46;<span class="elsevierStyleSup">3</span> During hypocalcemia&#44; increased PTH secretion restores serum calcium to normal&#46; Besides its bone effect&#44; PTH increases renal calcium reabsorption and calcitriol production enhancing gut absorption of calcium&#46; However&#44; the instantaneous effect and that of greatest magnitude is attributed to calcium efflux from bone&#46;</p><p class="elsevierStylePara">Not often discussed is the role of a calcium gradient with greater mobilization of calcium from bone in hypocalcemia than in hypercalcemia&#46; When increasing PTH doses were infused in parathyroidectomized rats&#44; the serum calcium response was curvilinear with a steeper response between 5 and 10mg&#47;dL than at higher values &#40;Figure 1 A&#41; showing that the calcium gradient in hypercalcemia reduced the calcemic action of PTH&#46;<span class="elsevierStyleSup">4</span> The effect of infused PTH on serum phosphorus was also curvilinear with a steeper decrease at lower PTH doses &#40;Figure 1 B&#41;&#46; These results show an important role for a calcium gradient in mobilizing calcium from bone with a possible complementary effect for phosphorus lowering&#46;</p><p class="elsevierStylePara">Interestingly&#44; even in the absence of PTH&#44; calcium efflux from bone does occur in response to reductions in serum calcium&#46; In parathyroidectomized&#44; hypocalcemic rats&#44; the rate of recovery from ethylene glycol tetraacetic acid &#40;EGTA&#41; induced lowering of serum calcium to baseline hypocalcemic values was similar to that in parathyroid-intact rats EGTA-induced hypocalcemia to normocalcemia&#46;<span class="elsevierStyleSup">5</span> In dogs&#44; recovery from hypocalcemia was shown to be more rapid in young than in adult dogs&#44; probably due to a greater exchangeable pool of bone calcium in young animals&#46;<span class="elsevierStyleSup">6</span> Results from animal studies suggest that the increase in calcium is bone derived&#46;<span class="elsevierStyleSup">5&#44;7&#44;8</span></p><p class="elsevierStylePara">What is the short-term role of PTH and does PTH play a role in maintaining the constancy of serum calcium throughout the day&#63; Clinical studies have shown that daily variations in serum calcium values are greater in hypoparathyroid patients than in normal humans and are affected more by variations in dietary calcium and phosphorus&#46;<span class="elsevierStyleSup">9</span> A possible explanation is that during fasting&#44; the normal human is better able to extract calcium from bone and during feeding to better deposit calcium into bone to maintain a constant serum calcium concentration&#46;<span class="elsevierStyleSup">10&#44;11</span></p><p class="elsevierStylePara">In the early 1990s&#44; D&#8217;Amour and associates showed that during PTH stimulation from hypocalcemia&#44; the ratio of intact PTH to carboxy &#40;C&#41;-PTH fragments increased and during PTH suppression from hypercalcemia&#44; the ratio of intact PTH to C-PTH fragments decreased&#46;<span class="elsevierStyleSup">12</span> Subsequently&#44; the same authors showed that besides 1-84 PTH&#44; the assay for intact PTH also detected non 1-84 PTH large truncated amino-terminal fragments of which 7-84 PTH was the prototype&#46;<span class="elsevierStyleSup">13</span> In normal humans&#44; non 1-84 PTH large truncated amino-terminal fragments represent about 20&#37; of measured intact PTH&#44; but in renal failure&#44; these same fragments account for approximately 50&#37;&#46;<span class="elsevierStyleSup">12&#44;13</span> Interest increased when the 7-84 PTH fragment was shown to inhibit the calcemic action of 1-84 and 1-34 PTH&#46;<span class="elsevierStyleSup">8&#44;14</span> The action of 7-84 PTH is most likely mediated by its binding to the C-PTH receptor present on osteocytes and other cells of osteoblast lineage including lining cells on the bone surface&#46;<span class="elsevierStyleSup">15&#44;16</span> Moreover&#44; the osteocyte has important signaling functions to the bone surface&#46;<span class="elsevierStyleSup">17</span> Finally&#44; 7-84 PTH may internalize the PTH1 receptor in selected target cells in kidney and bone<span class="elsevierStyleSup">18</span> and also reduce calcitriol production&#46;<span class="elsevierStyleSup">19</span> Thus&#44; in advanced renal failure&#44; greatly increased 7-84 PTH could decrease the calcemic response to PTH in absence of hypercalcemia&#46;<span class="elsevierStyleSup">2</span></p><p class="elsevierStylePara">An intriguing observation in azotemic rats is that parathyroidectomy completely restored the calcemic response to PTH even though hyperphosphatemia was present before the 1-34 PTH infusion &#40;Figure 2 A&#41;&#46;<span class="elsevierStyleSup">20</span> Before the PTH infusion&#44; rats were maintained on a high calcium diet for more than two weeks after parathyroidectomy&#46; It is possible that the total absence of 7-84 PTH or other PTH fragments resulted in the greater response to PTH&#46; Other possibilities not mutually exclusive include that the total absence of PTH resulted in an upregulation of the PTH1 receptor&#44; hypocalcemia lessened the gradient driving mineralization increasing the exchangeable bone pool of calcium&#44; and the high calcium diet resulted in expansion of the exchangeable bone pool of calcium&#46;</p><p class="elsevierStylePara">The size of the exchangeable pool of bone calcium differs among the different forms of renal osteodystrophy in CKD&#46; In studies in hemodialysis patients performed in the 1980s and 1990s&#44; we used low and high calcium dialysates during hemodialysis to induce hypo- and hypercalcemia to evaluate PTH stimulation and suppression&#46; The capacity to defend against the development of hypocalcemia and hypercalcemia was much greater in hemodialysis patients with high &#40;osteitis fibrosa&#41; than with low bone turnover &#40;adynamic bone or osteomalacia&#41;&#46;<span class="elsevierStyleSup">21-23</span> A similarly expanded pool of bone calcium and increased calcium efflux from bone has been shown in dialysis patients with osteitis fibrosa studied with calcium isotopes&#46;<span class="elsevierStyleSup">24</span> Moreover&#44; in osteitis fibrosa&#44; bone is deposited in a woven rather than the usual lamellar pattern&#46; The abnormal woven bone may bind or incorporate calcium less well&#46;<span class="elsevierStyleSup">25</span> Also&#44; trabecular bone volume and thickness were greater in dialysis patients with high than low bone turnover suggesting that greater bone mass is present for calcium exchange in the former&#46;<span class="elsevierStyleSup">25</span> A summary of the discussion on PTH and bone is provided in Table 1&#46;</p><p class="elsevierStylePara"><span class="elsevierStyleBold">&#160;</span></p><p class="elsevierStylePara"><span class="elsevierStyleBold">PHOSPHATE</span><span class="elsevierStyleBold">&#160;</span></p><p class="elsevierStylePara">&#160;</p><p class="elsevierStylePara">Phosphate is an important factor in evaluating the calcemic response to PTH&#46; Long ago Albright showed that the first action of infused parathyroid extract was phosphaturia followed by lowering serum phosphorus before a calcemic effect developed&#46;<span class="elsevierStyleSup">26</span> In the 1960s&#44; Raisz showed in an ex-vivo study that increasing the phosphate concentration in the medium reduced calcium efflux from bone both in the absence and presence of PTH&#46;<span class="elsevierStyleSup">27</span> Phosphate also modifies calcium efflux from bone in animal and human studies&#46;<span class="elsevierStyleSup">28-30</span> In parathyroidectomized rats given a normal phosphate diet and a fixed replacement dose of PTH sufficient to maintain normal serum calcium and phosphorus values&#44; changing to a high phosphate diet resulted in hypocalcemia and hyperphosphatemia&#46;<span class="elsevierStyleSup">29</span> In young&#44; healthy subjects given continuous intravenous phosphate infusion for 7 days&#44; hypocalcemia and hyperphosphatemia developed until a three-fold increase in PTH increased phosphate excretion by five-fold and normalized serum calcium and phosphorus&#46;<span class="elsevierStyleSup">28</span> Conversely&#44; phosphate depletion induces hypercalcemia despite marked suppression of PTH&#46;<span class="elsevierStyleSup">31</span> Even high dose bisphosphonate in phosphate-depleted rats did not prevent hypercalcemia despite producing toxic effects in osteoclasts and a marked decrease in the osteoblast surface&#46;<span class="elsevierStyleSup">31</span> These results support the concept that an osteoclast independent effect may be important for bone efflux of calcium&#46;<span class="elsevierStyleSup">32&#44;33</span> Finally&#44; bisphosphonate treatment in phosphate-depleted rats increased urine calcium excretion more than in phosphate-depleted rats not receiving bisphosphonates suggesting that decreased calcium influx to bone may be a factor&#46;</p><p class="elsevierStylePara">In renal failure&#44; many studies have shown that phosphate loading or hyperphosphatemia decreases the calcemic response to PTH&#46;<span class="elsevierStyleSup">34-36</span> When 5&#47;6 nephrectomy was performed in parathyroidectomized rats&#44; a replacement dose of 1-34 PTH sufficient to maintain normal serum calcium and phosphorus values in parathyroidectomized rats with normal renal function failed to maintain normal serum calcium and phosphorus levels in renal failure&#46;<span class="elsevierStyleSup">29</span> Even though dietary phosphate was unchanged&#44; the phosphate burden was presumably increased because of decreased renal excretion of phosphate&#46;<span class="elsevierStyleSup">29</span> Conversely&#44; dietary phosphate restriction in renal failure decreases PTH while maintaining the serum calcium value&#46; In essence&#44; less PTH is needed to maintain the serum calcium concentration&#46;<span class="elsevierStyleSup">37-40</span> Even in patients with advanced CKD&#44; dietary phosphate restriction decreases PTH while maintaining normal serum calcium values&#46;<span class="elsevierStyleSup">41&#44;42</span> A summary of the discussion of the effect of phosphate on serum calcium and bone is provided in Table 2&#46;</p><p class="elsevierStylePara"><span class="elsevierStyleBold">&#160;</span></p><p class="elsevierStylePara"><span class="elsevierStyleBold">VITAMIN D</span><span class="elsevierStyleBold">&#160;</span></p><p class="elsevierStylePara">&#160;</p><p class="elsevierStylePara">The effect of vitamin D on the exchangeable pool of bone calcium is more difficult to define than that of phosphate&#46; Under some circumstances&#44; 1&#44;25 vitamin D &#40;1&#44;25D&#41; appears to enhance&#44; but in others to reduce calcium efflux from bone&#46; In vitamin D intoxication&#44; studies have suggested that calcium efflux from bone is increased&#46;<span class="elsevierStyleSup">43-48</span> In vitro and in vivo studies have shown that calcitriol increases osteoclast activity&#46;<span class="elsevierStyleSup">49-51</span> In a case report of vitamin D intoxication&#44; bone histomorphometry showed an increase in osteoclasts with a reduction in osteoblasts&#44; an increased osteoid surface and volume&#44; and decreased mineralization&#46;<span class="elsevierStyleSup">45</span> Studies in the chick also found that vitamin D intoxication impaired bone formation&#46;<span class="elsevierStyleSup">52&#44;53</span> The effects on osteoid volume and bone formation suggest that calcium influx to bone may be decreased in vitamin D intoxication&#46; Both corticosteroids and bisphosphonates effectively treat hypercalcemia in vitamin D intoxication&#46;<span class="elsevierStyleSup">43-45</span> However&#44; in different settings&#44; bisphosphonate treatment did not change the rate of recovery from EDTA-induced hypocalcemia in osteoporotic patients<span class="elsevierStyleSup">54</span> or prevent hypercalcemia in rats on a phosphate depleted diet perhaps suggesting different mechanisms&#46;<span class="elsevierStyleSup">31</span></p><p class="elsevierStylePara">In a recent study in intestinal-specific VDR-null mice in which calcium absorption is unresponsive to 1&#44;25D&#44; insights into the calcemic and bone effects of 1&#44;25D during calcium deficiency were provided&#46;<span class="elsevierStyleSup">55</span> In this mouse model&#44; markedly elevated endogenous 1&#44;25D levels maintained serum calcium normal by increasing calcium efflux from bone while bone mineralization was inhibited resulting in hyperosteoidosis and a decrease in bone mass&#46; The activation of bone resorption by 1&#44;25D in calcium deficient intestinal-specific VDR-null mice was osteoblast mediated&#46; In systemic VDR-receptor null mice&#44; bone abnormalities were prevented when calcium repletion was achieved with a high calcium&#47;lactose diet&#46;<span class="elsevierStyleSup">56</span> In the intestinal-specific null model&#44; although PTH values were elevated&#44; the authors concluded that because markedly elevated PTH did not prevent hypocalcemia in systemic VDR null mice&#44; 1&#44;25D played a critical role in increasing calcium efflux from bone in intestinal-specific VDR-null mice&#46; Finally&#44; the calcemic bone actions of 1&#44;25D were blocked by bisphosphonates&#46; The authors concluded that high 1&#44;25D levels in calcium deficiency&#58; 1&#41; increase calcium efflux from bone&#59; 2&#41; decrease calcium influx to bone by inhibiting bone mineralization&#59; and 3&#41; both 1&#41; and 2&#41; act to maintain serum calcium&#44; but decrease bone mass&#46;</p><p class="elsevierStylePara">In intestinal-specific VDR null mice&#44; a marked elevation in 1&#44;25D increased calcium efflux from bone&#46;<span class="elsevierStyleSup">55</span> But a major question is whether such a mechanism is applicable in vitamin D deficiency&#63; In dogs placed on a vitamin D and calcium deficient diet&#44; 25D values decreased rapidly&#44; but 1&#44;25D values tripled by 24 weeks and remained greater than normal at 36 weeks before subsequently declining below normal&#46;<span class="elsevierStyleSup">57</span> Ionized calcium values remained normal until 24 weeks before marginally decreasing at 36 weeks&#46; Thus&#44; despite vitamin D and calcium deficiency&#44; serum calcium values were maintained normal for a prolonged time as 1&#44;25D and PTH values tripled during the first 24 weeks&#46; An elevated 1&#44;25D and PTH together with hypophosphatemia contributed to the prolonged maintenance of serum calcium&#46; The marked 1&#44;25D increase during the first 24 weeks could have helped maintain serum calcium by enhancing calcium efflux from bone while also decreasing calcium influx to bone&#46; In humans with established vitamin D deficiency&#44; studies have shown that treatment with vitamin D or 25D increases 1&#44;25D values to 3 to 4 times greater than normal&#46;<span class="elsevierStyleSup">58-60</span> Thus&#44; it is plausible in vitamin D deficiency that high 1&#44;25D values have an important skeletal effect in maintaining serum calcium during its evolution and increasing serum calcium during initial treatment with vitamin D&#46; Also&#44; in evolving vitamin D deficiency&#44; high 1&#44;25D values perhaps together with PTH-induced decreases in serum phosphorus potentially contribute to the development of osteomalacia&#46;</p><p class="elsevierStylePara">In renal failure&#44; several studies have shown that acute calcitriol administration increases the calcemic response to PTH&#46;<span class="elsevierStyleSup">61&#44;62</span> In one study&#44; high dose PTH was infused for 48 hours while calcitriol was administered to rats on a calcium free diet&#46;<span class="elsevierStyleSup">20</span> Thus&#44; neither calcitriol nor hypercalcemia could modify PTH levels&#46; Calcitriol administration enhanced the calcemic response to PTH in azotemic rats on a low phosphate diet and in both normal and azotemic rats on a high phosphate diet &#40;Figure 2 B&#41;&#46;<span class="elsevierStyleSup">20</span></p><p class="elsevierStylePara">Two studies in dialysis patients are of particular interest because long-term calcitriol treatment decreased the bone response to PTH&#46;<span class="elsevierStyleSup">63&#44;64</span> In one study&#44; 14 children on peritoneal dialysis with biopsy-proven osteitis fibrosa were treated with high dose calcitriol for 12 months with dose adjustments for hypercalcemia and hyperphosphatemia&#46;<span class="elsevierStyleSup">63</span> The average calcitriol dose was 1&#46;25&#181;g thrice weekly&#46; The dialysate calcium concentration was 3&#46;5meq&#47;L and calcium carbonate was the primary phosphate binder&#46; A repeat bone biopsy performed after one year showed that 12 patients developed normal or adynamic bone and in 6 patients the bone formation rate decreased despite no change in PTH values&#46; The latter result suggests that calcitriol treatment may have decreased the bone formation rate similar to results seen in intestinal-specific VDR null mice&#46;<span class="elsevierStyleSup">55</span> A positive calcium balance from calcium carbonate and high dialysate calcium concentration could also have contributed&#46;</p><p class="elsevierStylePara">In the other study&#44; a hemodialysis patient with markedly elevated intact PTH &#40;1912pg&#47;mL&#44; Nichols assay&#41; and serum alkaline phosphatase &#40;1512IU&#47;L&#41; was studied before calcitriol treatment&#44; after 6 weeks of 2&#181;g intravenous calcitriol thrice weekly&#44; and 6 weeks after calcitriol was stopped&#46;<span class="elsevierStyleSup">64</span> Studies consisted of separate dialysis sessions with low &#40;1meq&#47;L&#41; and high &#40;4meq&#47;L&#41; calcium dialysate to determine maximal PTH secretion and suppression respectively&#46; Despite 6 weeks of calcitriol treatment&#44; predialysis serum calcium and PTH values were similar to pretreatment values &#40;Figure 3 A and Figure 3 B&#41; as were serum phosphorus and alkaline phosphatase values&#46; The maximal PTH value during induction of hypocalcemia was similar approaching 4000pg&#47;mL during both low calcium dialysis studies &#40;Figure 3 B&#41;&#46; However&#44; during the low calcium dialysis in the first study&#44; serum calcium&#44; which started at 10&#46;8mg&#47;dL&#44; only decreased to 9&#46;4mg&#47;dL at 180 minutes&#46; After calcitriol treatment&#44; despite a similar starting serum calcium concentration&#44; serum calcium decreased to less than 8mg&#47;dL after only 15 minutes of dialysis &#40;Figure 3A&#41;&#46; Studies with the high calcium dialysate showed marked differences in bone buffering of calcium&#46; Before calcitriol treatment&#44; serum calcium slowly increased to 11&#46;8mg&#47;dL at 180 minutes&#46; However&#44; after calcitriol treatment&#44; serum calcium increased to 12&#46;2mg&#47;dL after only 15 minutes &#40;Figure 3 C&#41;&#46; PTH suppression was similar &#40;Figure 3 D&#41;&#46; When dialysis studies were repeated after calcitriol had been stopped for 6 weeks&#44; results were similar to those before calcitriol was started &#40;Figure 3&#41;&#46; Thus&#44; in this hemodialysis patient with severe secondary hyperparathyroidism and an enhanced capacity to defend against the induction of hypocalcemia and hypercalcemia&#44; calcitriol treatment for 6 weeks dramatically decreased calcium efflux from and influx to bone&#46;</p><p class="elsevierStylePara">In both cited studies&#44; a decreased bone&#47;calcemic response to PTH was seen&#46; In the second study&#44; the exchangeable pool of bone calcium was seemingly greatly reduced by calcitriol treatment despite no change in PTH secretion&#46; In the first study it could be argued that the decrease in osteoblasts and osteoclasts&#44; which reflects bone remodeling&#44; is different from an evaluation of bone efflux and influx of calcium&#46; However&#44; the marked reduction in bone formation rate together with the decreased cellular activity seen after calcitriol treatment is also a likely indicator of a decreased exchangeable pool of bone calcium&#46;</p><p class="elsevierStylePara">How does the vitamin D&#47;calcium biochemical profile differ between dialysis patients receiving high doses of calcitriol and patients with vitamin D intoxication&#63; In dialysis patients&#44; calcitriol treatment reduced calcium efflux from bone during a low calcium dialysis while in patients with vitamin D intoxication increased calcium efflux from bone is reported to be a major contributor to hypercalcemia&#46; In vitamin D intoxication&#44; hypercalcemia is persistent with suppressed PTH&#46; In dialysis patients treated with calcitriol&#44; if monitored diligently the serum calcium concentration is normal while the high PTH value generally decreases&#44; but remains elevated&#46; In the study in which calcitriol was given thrice weekly&#44; measured serum calcitriol values were in the normal range before subsequent dosing&#44;<span class="elsevierStyleSup">63</span> but high 1&#44;25D values would be expected immediately after calcitriol dosing&#46;<span class="elsevierStyleSup">65</span> In dialysis patients&#44; 25OHD values are often less than the desired goal&#44; 30ng&#47;mL&#46; Conversely&#44; in vitamin D intoxication&#44; 25OHD values are markedly elevated while serum calcitriol values are often but not always increased&#46;<span class="elsevierStyleSup">66-68</span></p><p class="elsevierStylePara">How does vitamin D affect calcium efflux from bone in vitamin D intoxication&#63; In vitamin D intoxication&#44; longstanding hypercalcemia leads to reduced renal function further exacerbating hypercalcemia&#44; which may act to drive calcium into bone&#46; Treatment with normal saline improves renal function by correcting the decreased extracellular volume&#44; which in turn&#44; increases renal excretion of calcium lowering serum calcium&#46; As a result&#44; the supersaturated bone releases more calcium for several reasons&#46;<span class="elsevierStyleSup">43-45</span> These include an increasing calcium gradient between bone and blood&#44; reduced serum phosphorus from improved renal function&#44; and increased PTH secretion from a reduction in serum calcium&#46; Such actions could account for the enhanced calcium release from bone reported in vitamin D intoxication&#46; Finally&#44; suggesting that increased bone calcium deposition may occur during the hypercalcemia of vitamin D intoxication is the report of increased bone mineral density in osteoporotic patients with vitamin D intoxication&#46;<span class="elsevierStyleSup">48</span></p><p class="elsevierStylePara">Why did high dose calcitriol treatment decrease the exchangeable pool of bone calcium in the dialysis patient with severe secondary hyperparathyroidism&#63; In dialysis patients with osteitis fibrosa&#44; there are abundant osteoblasts in which the VDR can be activated to generate osteoclasts and increase bone resorption&#46; However&#44; calcitriol administration decreased the exchangeable pool of bone calcium&#46; In both cited studies in patients with osteitis fibrosa&#44; a calcium-replete environment was present due to high dialysate calcium&#44; the use of calcium-based phosphate binders&#44; and intermittent or continual hypercalcemia&#46;<span class="elsevierStyleSup">63&#44;64</span> In contrast&#44; in studies in intestinal-specific VDR-null mice in which 1&#44;25D-mediated increased bone resorption and decreased bone formation were seen&#44; calcium depletion was present&#46;<span class="elsevierStyleSup">55</span> However&#44; when the VDR-null mouse was calcium replete&#44; increased bone resorption and decreased bone formation were prevented&#46;<span class="elsevierStyleSup">56</span> Other important differences are present between dialysis patients with osteitis fibrosa and intestinal-specific VDR null mice&#47;patients with vitamin D deficiency&#46; In the dialysis patients&#44; calcitriol administration was intermittent in contrast to continuously elevated&#44; endogenous 1&#44;25D values&#46; Although PTH values are increased in all the cited conditions&#44; the calcium and phosphorus status are quite different&#46; In the dialysis patients&#44; hypercalcemia and hyperphosphatemia are present while in intestinal specific null mice and vitamin D deficient patients&#44; hypocalcemia and hypophosphatemia prevail&#46; In the reported dialysis patient&#44;<span class="elsevierStyleSup">64</span> increases in serum calcium were not seen with calcitriol treatment&#46; However&#44; whether increased bone buffering of calcium <span class="elsevierStyleBold">not only</span> prevented hypercalcemia&#44; but also drove the mineralization process decreasing the exchangeable pool of bone calcium is an interesting possibility&#46; A summary of the discussion on 1&#44;25D and bone is provided Table 3&#46;</p><p class="elsevierStylePara">A final point of discussion is the effect of both 19-nor-1&#44;25-dihydroxy-vitamin D<span class="elsevierStyleInf">2 </span>&#40;paricalcitol&#41;&#44; an analog of 1&#44;25D&#44; and the calcimimetic&#44; cinacalcet&#44; on bone resorption and serum calcium&#46; Both agents have been used for the past 10 to 15 years for the treatment of hyperparathyroidism in dialysis patients&#46; To achieve similar PTH suppression&#44; the dose ratio of paricalcitol to calcitriol used has been 3 or 4&#58;1&#46; Most&#44; but not all in vitro and animal studies have shown that the calcemic effect of paricalcitol is less than that of calcitriol&#46;<span class="elsevierStyleSup">69-71</span> In studies in dialysis patients&#44; paricalcitol suppresses PTH secretion at least as well if not better than calcitriol<span class="elsevierStyleSup">72</span> and may also have less calcemic effect&#46;<span class="elsevierStyleSup">72&#44;73</span> In studies in uremic rats&#44; less vascular calcification has been seen with paricalcitol than with calcitriol<span class="elsevierStyleSup">74</span> and calcimimetics appear to be even more effective in preventing vascular calcification&#46;<span class="elsevierStyleSup">75</span> A summary of the discussion on 1&#44;25D and bone is provided Table 3&#46;</p><p class="elsevierStylePara">Calcimimetics are an effective treatment for hyperparathyroidism in the uremic animals and dialysis patient&#46; Because of its calcimimetic activity&#44; hypocalcemia generally develops during treatment&#46; Several studies in rats have evaluated the effect of calcimimetics on bone&#46;<span class="elsevierStyleSup">76-78</span> Two studies in rats used a 5&#47;6 nephrectomy model<span class="elsevierStyleSup">76&#44;78</span> and the other an adenine model of uremia&#46;<span class="elsevierStyleSup">77</span> The latter model results in much more severe renal failure&#44; hyperparathyroidism&#44; hypocalcemia and hyperphosphatemia than the former model&#46; In one of the 5&#47;6 nephrectomy models&#44; a high phosphate diet was used<span class="elsevierStyleSup">78</span> and in the other a relatively high calcium diet was used&#46;<span class="elsevierStyleSup">76</span> Moreover&#44; different calcimimetics were used in the three studies&#46; In all the studies&#44; serum calcium and PTH decreased&#44; but the magnitude of the reduction in PTH differed&#46; Changes in bone histology also differed considerably among the studies&#46; In another study simulating primary hyperparathyroidism&#44; the bone response to a two week continuous infusion of PTH was evaluated in mice with either a PTH gene knockout or PTH and calcium sensing receptor gene knockout &#40;double knockout&#41;&#46; The mice had normal renal function&#46; The absence of the calcium sensing receptor reduced the bone effects of PTH in this model of primary hyperparathyroidism&#46;<span class="elsevierStyleSup">79</span></p><p class="elsevierStylePara"><span class="elsevierStyleBold">&#160;</span></p><p class="elsevierStylePara"><span class="elsevierStyleBold">UREMIC TOXINS</span><span class="elsevierStyleBold">&#160;</span></p><p class="elsevierStylePara">&#160;</p><p class="elsevierStylePara">A variety of factors which could potentially contribute to skeletal resistance to PTH are altered in CKD&#46; Exposure of osteoblast-like cells to uremic serum affects the number and function of these cells including suppressed mitogenesis and IL-6 release and reduced PTHR expression&#46;<span class="elsevierStyleSup">80-82</span> In CKD&#44; levels of inhibitory and stimulatory insulin growth factor binding proteins 4 and 5 respectively&#44; are altered and may contribute to abnormal osteoblast proliferation&#46;<span class="elsevierStyleSup">80</span> Osteoprotegerin&#44; a decoy receptor of NF-kB ligand &#40;RANKL&#41;&#44; accumulates as renal failure progresses blocking osteoclast activation by PTH&#46;</p><p class="elsevierStylePara">The concentration of absorbed products of intestinal microbial flora increase as renal function declines&#46; These products&#44; particularly indoxyl sulfate and p-cresol sulfate&#44; may alter bone metabolism and contribute to bone resistance to PTH&#46; Indole&#44; formed by gastrointestinal bacteria during breakdown of tryptophan&#44; is metabolized to indoxyl sulfate which is transported by an organic anion transporter into osteoblasts where it induces oxidative stress&#46; Exposure of osteoblast cells in culture to indoxyl sulfate inhibits PTH-induced cyclic AMP generation and downregulates PTHR expression&#44; an effect partially inhibited by the organic anion transporter inhibitor&#44; probenicid&#46;<span class="elsevierStyleSup">83</span> Elevated indoxyl sulfate values in hemodialysis patients inversely correlate with markers of bone formation&#46;<span class="elsevierStyleSup">84</span> Furthermore&#44; in a rat model of adynamic bone in which elevated indoxyl sulfate values were present&#44; administration of the oral absorbent&#44; AST-120&#44; lowered indoxyl sulfate values and increased bone formation&#46;<span class="elsevierStyleSup">85</span></p><p class="elsevierStylePara">Indoxyl sulfate and p-cresol sulfate&#44; a gut microbial product produced from metabolism of tyrosine&#44; may indirectly alter PTH action on bone through the FGF23-klotho axis&#46; Klotho blood values are low in CKD while FGF23 values are markedly elevated&#46; In a mouse model of CKD and in isolated kidney cells&#44; both indoxyl sulfate and p-cresol sulfate suppressed klotho production by epigenetic modification of the klotho gene&#46;<span class="elsevierStyleSup">86</span> These toxins stimulate expression of DNA methyltransferase 1 which hypermethylates the klotho gene suppressing gene expression&#46; A deficiency of klotho as exemplified by the klotho knockout mouse results in elevated values of serum calcium&#44; phosphorus and 1&#44;25D&#44; ectopic calcification including extensive vascular calcification&#44; and osteoporosis with decreases in osteoblasts and osteoclasts&#46;<span class="elsevierStyleSup">87</span> FGF23 values are markedly elevated in the klotho knockout model similar to that in CKD&#46; Thus&#44; reduced levels of klotho in CKD&#44; perhaps at least in part due to uremic toxins&#44; could alter the bone response to PTH via hyperphosphatemia and&#47;or extremely elevated FGF23 levels found in CKD&#46;<span class="elsevierStyleSup">88</span> The potential role of uremic toxins on bone is summarized in Table 4&#46;</p><p class="elsevierStylePara">A summary of the many factors affecting calcium efflux from bone discussed in our review is shown in Figure 4&#46; Many unanswered questions exist about the exchangeable pool of bone calcium&#46; These include 1&#41; how PTH mobilizes calcium from bone&#44; 2&#41; what is the contribution of osteoclast-mediated calcium efflux in contrast to shifts across the quiescent bone surface&#44; 3&#41; how after induced hypocalcemia&#44; serum calcium is restored to baseline values in parathyroidectomized animals&#44; 4&#41; how parathyroidectomy restores the calcemic response to PTH to normal in the azotemic rat&#44; 5&#41; how phosphate modifies calcium efflux from bone&#44; 6&#41; why bisphosphonate treatment reduces calcium efflux from bone in vitamin D intoxication&#44; but not in phosphate depletion&#44; 7&#41; how calcitriol treatment decreases the exchangeable pool of bone calcium in dialysis patients with osteitis fibrosa&#44; and 8&#41; the role of uremic toxins on the exchangeable pool of bone calcium&#46; Much remains to be learned about the role of bone in the regulation of serum calcium&#46;</p><p class="elsevierStylePara">&#160;</p><p class="elsevierStylePara"><span class="elsevierStyleBold">ACKNOWLEDGMENTS</span></p><p class="elsevierStylePara">&#160;</p><p class="elsevierStylePara">M&#46;R&#46; has received grant support from the Instituto Carlos III &#40;FIS 07&#47;0287&#44; FIS 07&#47;0315&#44; 010&#47;1311&#41;&#44; Consejer&#237;a de Salud &#40;JA 0127&#47;2008&#41;&#44; a UE Grant from Framework Programme 7 Syskid &#40;FP7-241544&#41;&#44; and Consejer&#237;a de Innovaci&#243;n&#44; Ciencia y Empresa &#40;CTS-5205 and CTS-170&#41; of Junta de Andaluc&#237;a&#46;</p><p class="elsevierStylePara">&#160;</p><p class="elsevierStylePara"><span class="elsevierStyleBold">DISCLOSURES</span></p><p class="elsevierStylePara">&#160;</p><p class="elsevierStylePara">B&#46;L and A&#46;F&#46; have no disclosures&#46; M&#46;R&#46; has received grants from Amgen and Fresenius and lecture fees from Amgen&#44; Abbott&#44; Shire and Fresenius&#46;</p><p class="elsevierStylePara">&#160;</p><p class="elsevierStylePara"><span class="elsevierStyleBold">Conflicts of interest</span></p><p class="elsevierStylePara">&#160;</p><p class="elsevierStylePara">The authors declare that they have no conflicts of interest related to the contents of this article&#46;</p><p class="elsevierStylePara"><a href="grande&#47;12379&#95;19904&#95;53551&#95;en&#95;12379&#95;t1&#46;jpg" class="elsevierStyleCrossRefs"><img src="12379_19904_53551_en_12379_t1.jpg" alt="Parathyroid hormone and calcium efflux from bone"></img></a></p><p class="elsevierStylePara">Table 1&#46; Parathyroid hormone and calcium efflux from bone</p><p class="elsevierStylePara"><a href="grande&#47;12379&#95;19904&#95;53552&#95;en&#95;12379&#95;t2&#46;jpg" class="elsevierStyleCrossRefs"><img src="12379_19904_53552_en_12379_t2.jpg" alt="Phosphorus and calcium efflux from bone"></img></a></p><p class="elsevierStylePara">Table 2&#46; Phosphorus and calcium efflux from bone</p><p class="elsevierStylePara"><a href="grande&#47;12379&#95;19904&#95;53553&#95;en&#95;12379&#95;t3&#46;jpg" class="elsevierStyleCrossRefs"><img src="12379_19904_53553_en_12379_t3.jpg" alt="Calcitriol and calcium efflux from bone"></img></a></p><p class="elsevierStylePara">Table 3&#46; Calcitriol and calcium efflux from bone</p><p class="elsevierStylePara"><a href="grande&#47;12379&#95;19904&#95;53554&#95;en&#95;12379&#95;t4&#46;jpg" class="elsevierStyleCrossRefs"><img src="12379_19904_53554_en_12379_t4.jpg" alt="Uremic toxins and calcium efflux from bone"></img></a></p><p class="elsevierStylePara">Table 4&#46; Uremic toxins and calcium efflux from bone</p><p class="elsevierStylePara"><a href="grande&#47;12379&#95;19904&#95;53555&#95;en&#95;12379&#95;f1&#46;jpg" class="elsevierStyleCrossRefs"><img src="12379_19904_53555_en_12379_f1.jpg" alt="Serum calcium and phosphorus response to PTH dosing in parathyroidectomized rats&#46;"></img></a></p><p class="elsevierStylePara">Figure 1&#46; Serum calcium and phosphorus response to PTH dosing in parathyroidectomized rats&#46;</p><p class="elsevierStylePara"><a href="grande&#47;12379&#95;19904&#95;53557&#95;en&#95;12379&#95;f2&#95;copy1&#46;jpg" class="elsevierStyleCrossRefs"><img src="12379_19904_53557_en_12379_f2_copy1.jpg" alt="Changes in the serum level of calcium and phosphorus during PTH infusion for 48 hours&#46;"></img></a></p><p class="elsevierStylePara">Figure 2&#46; Changes in the serum level of calcium and phosphorus during PTH infusion for 48 hours&#46;</p><p class="elsevierStylePara"><a href="grande&#47;12379&#95;19904&#95;53558&#95;en&#95;12379&#95;f3&#46;jpg" class="elsevierStyleCrossRefs"><img src="12379_19904_53558_en_12379_f3.jpg" alt="Serum calcium and PTH response to low and high calcium dialysate before&#44; during and after calcitriol &#40;CTR&#41; treatment&#46;"></img></a></p><p class="elsevierStylePara">Figure 3&#46; Serum calcium and PTH response to low and high calcium dialysate before&#44; during and after calcitriol &#40;CTR&#41; treatment&#46;</p><p class="elsevierStylePara"><a href="grande&#47;12379&#95;19904&#95;53560&#95;en&#95;12379&#95;f4&#46;jpg" class="elsevierStyleCrossRefs"><img src="12379_19904_53560_en_12379_f4.jpg" alt="Schematic of factors affecting calcium efflux from bone are shown&#46;"></img></a></p><p class="elsevierStylePara">Figure 4&#46; Schematic of factors affecting calcium efflux from bone are shown&#46;</p>"
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        "resumen" => "<p class="elsevierStylePara">El hiperparatiroidismo se desarrolla en la enfermedad renal cr&#243;nica &#40;ERC&#41;&#46; La disminuci&#243;n de la respuesta calc&#233;mica a la hormona paratiroidea &#40;PTH&#41; contribuye al desarrollo de hiperparatiroidismo y es probable que se deba a una reducci&#243;n de la emisi&#243;n de calcio de los huesos&#46; Entre los factores que contribuyen a la disminuci&#243;n de la respuesta calc&#233;mica a la PTH en la ERC se encuentran&#58; 1&#41; la hiperfosfatemia&#59; 2&#41; la disminuci&#243;n del calcitriol s&#233;rico&#59; 3&#41; la desensibilizaci&#243;n del receptor PTHR1&#59; 4&#41; la presencia de fragmentos de gran tama&#241;o de los extremos aminoterminales de la hormona paratiroidea que act&#250;an en el receptor carboxi-PTH y 5&#41; las toxinas ur&#233;micas&#46; Asimismo&#44; la administraci&#243;n prolongada de una dosis elevada de calcitriol podr&#237;a disminuir la reserva intercambiable de calcio independiente de la hormona paratiroidea&#46; El objetivo de esta revisi&#243;n es facilitar la comprensi&#243;n de c&#243;mo afectan los factores mencionados anteriormente a la emisi&#243;n de calcio procedente del hueso en la ERC&#46; Como conclusi&#243;n&#44; a&#250;n queda mucho por aprender acerca del papel de los huesos en la regulaci&#243;n del calcio s&#233;rico&#46;</p>"
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        "resumen" => "<p class="elsevierStylePara">Hyperparathyroidism develops in chronic kidney disease &#40;CKD&#41;&#46; A decreased calcemic response to parathyroid hormone &#40;PTH&#41; contributes to the development of hyperparathyroidism and is presumed due to reduced calcium efflux from bone&#46; Contributing factors to the decreased calcemic response to PTH in CKD include&#58; 1&#41; hyperphosphatemia&#59; 2&#41; decreased serum calcitriol&#59; 3&#41; downregulation of the PTH1 receptor&#59; 4&#41; large&#44; truncated amino-terminal PTH fragments acting at the carboxy-PTH receptor&#59; and 5&#41; uremic toxins&#46; Also&#44; prolonged high dose calcitriol administration may decrease the exchangeable pool of bone calcium independent of PTH&#46; The goal of the review is to provide a better understanding of how the above cited factors affect calcium efflux from bone in CKD&#46; In conclusion&#44; much remains to be learned about the role of bone in the regulation of serum calcium&#46;</p>"
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Article information

ISSN: 20132514
Original language: English

DOI:10.3265/Nefrologia.pre2014.Jun.12379

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Year/Month	Html	Pdf	Total

2024 November	4	6	10
2024 October	158	60	218
2024 September	164	43	207
2024 August	211	69	280
2024 July	165	63	228
2024 June	227	61	288
2024 May	218	66	284
2024 April	182	46	228
2024 March	183	32	215
2024 February	228	40	268
2024 January	261	51	312
2023 December	329	54	383
2023 November	310	55	365
2023 October	295	41	336
2023 September	238	38	276
2023 August	237	38	275
2023 July	261	35	296
2023 June	247	24	271
2023 May	286	48	334
2023 April	224	13	237
2023 March	248	33	281
2023 February	263	29	292
2023 January	223	26	249
2022 December	195	44	239
2022 November	222	64	286
2022 October	169	65	234
2022 September	147	37	184
2022 August	160	67	227
2022 July	144	51	195
2022 June	184	44	228
2022 May	247	56	303
2022 April	271	68	339
2022 March	241	86	327
2022 February	256	54	310
2022 January	284	50	334
2021 December	172	58	230
2021 November	172	44	216
2021 October	246	51	297
2021 September	191	47	238
2021 August	197	45	242
2021 July	196	39	235
2021 June	191	28	219
2021 May	223	47	270
2021 April	562	99	661
2021 March	370	51	421
2021 February	351	39	390
2021 January	229	27	256
2020 December	238	29	267
2020 November	233	36	269
2020 October	143	28	171
2020 September	233	32	265
2020 August	180	27	207
2020 July	241	37	278
2020 June	198	28	226
2020 May	194	32	226
2020 April	247	45	292
2020 March	249	35	284
2020 February	252	31	283
2020 January	266	35	301
2019 December	307	43	350
2019 November	271	34	305
2019 October	368	37	405
2019 September	411	42	453
2019 August	326	40	366
2019 July	325	51	376
2019 June	311	47	358
2019 May	376	40	416
2019 April	357	59	416
2019 March	266	47	313
2019 February	127	39	166
2019 January	143	49	192
2018 December	330	54	384
2018 November	379	25	404
2018 October	470	36	506
2018 September	471	27	498
2018 August	322	28	350
2018 July	266	20	286
2018 June	297	12	309
2018 May	336	14	350
2018 April	322	11	333
2018 March	290	12	302
2018 February	208	11	219
2018 January	216	13	229
2017 December	201	10	211
2017 November	273	21	294
2017 October	239	16	255
2017 September	263	15	278
2017 August	271	22	293
2017 July	232	18	250
2017 June	306	23	329
2017 May	280	11	291
2017 April	198	16	214
2017 March	314	13	327
2017 February	405	31	436
2017 January	161	9	170
2016 December	326	21	347
2016 November	408	23	431
2016 October	369	30	399
2016 September	568	10	578
2016 August	666	34	700
2016 July	451	13	464
2016 June	283	0	283
2016 May	257	0	257
2016 April	241	0	241
2016 March	214	0	214
2016 February	196	0	196
2016 January	184	0	184
2015 December	242	0	242
2015 November	176	0	176
2015 October	167	0	167
2015 September	149	0	149
2015 August	151	0	151
2015 July	209	0	209
2015 June	87	0	87
2015 May	119	0	119
2015 April	21	0	21

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